《Assessment of introduced Kappaphycus Solieriaceae Rhodophyta species relationships in China with molecular markers》.pdfVIP

Acta Ocean01．Sin．，2013．V01．32，No．2，P59．65 DOI：10．1007／s13131-013-0277-5 http：／／www．hyxb．org．cn E-mail：hyxbe@263．net Assessmentofintroduced inChinawithmolecularmarkers speciesrelationships LIU Chenlinh，HUANG Xiaohan91，LIUJiangu02 1 Marine FirstInstitute Oceanic Center,The Administration， Ecological ofOceanography,State Qingdao266061，China 2Instituteof Chinese of 266071，China Oceanology,theAcademySciences，Qingdao Received23 June 20 2012 2011；acceptedFebruary @TheChinese and Berlin Society 2013 ofOceanographySpringer—VerlagHeidelberg Abstract to Due and of of morphologicalplasticitypaucitydiagnosticmorphologicalcharacters，the taxonomyKap— moreandmoreconfused埘mthe ofcommercialcultivation．Inthis paphycusgets expanding study,the of13strainsofintroduced inChinawasdefinedDNA phylogeneticrelationship Kappaphycusspecies using molecular as18S andcox2-cox3 resolutions markers，suchrDNA，rbcL obtainedthree spacerregion．The by differentmolecularmarkerswere cox2-cox3 and巾cL are compared：bothspacer region sequenceseligible in identificationof cox2-cox3 ismorevariablethanrbcL inter—species Kappaphycus,whereasspacerregion isseveral 18SrDNA itis100％identical sequence．Therebasepairs’discrepancyamong sequences，while