HPLC梯度洗脱法同时测定蜂胶牙痛酊中甲硝唑和丁香酚的含量_余小平.pdf

第 28卷 第 2期 Vol. 28 No. 2 2 0 1 0年 2月 CH INESE ARCH IVES OF TRAD IT OI NAL CH INESE M ED CI INE Feb. 2 0 1 0 HPLC 余小平,舒金富, 黄 华, 郑 明 (, 324002) : 目的:建立 HPLC梯度洗脱法同时测定蜂胶牙 酊中甲硝唑和 丁香酚的含量的新方法方法: HPLC 法,采用 AgilentEclipse DB- C ( 5Lm, 4. 6mm @ 150mm) 色谱柱, 流动相组成: A 相为甲醇, B相为水, 进行梯 18 1 度洗脱; 流速 1. 0mL# m in , 柱温: 28e , 二极管阵列检测器(DAD), 检测波长 293nm, 以外标法峰面积定量结 果: 甲硝唑 丁香酚与阴性空白样品溶液分离良好, 甲硝唑在 16. 26~ 162. 6L g 内呈良好的线性关系 ( r = 0. 999 5) , 丁香酚在 80. 16~ 801. 6L g内呈良好的线性关系( r = 1. 0000), 样品溶液在 24h内稳定, 平均加样回收率甲 硝唑为 101. 0%, RSD= 1. 06% ( n = 9), 丁香酚为 101. 0%, RSD= 0. 84% ( n = 9)结论: 该方法简便快速, 测定 结果准确可靠, 重现性好, 可用于蜂胶牙 酊的质量控制 : HPLC法; 蜂胶牙 酊; 蜂胶; 甲硝唑; 丁香酚; 梯度洗脱 : R284. 1 : A : 1673- 7717( 2010) 02- 0429- 03 Determ ination ofMetronidazoel and Eu enolum in Fen jiao Yaton D in by HPLC YU iao-ping, SHU Jin-fu, HUANG Hua, ZHENG M ing ( Quzhou Institute forDrug Control, Quzhou 324002, Zhejiang, China) Abstrac:t O bj ect iv e: To establish a HPLC method to determine the content of M etronidazole and Eugenolum in Fengjiao Yatong D ing. M ethod s: Agilent Eclipse DB- C column( 4. 6mm @ 150mm, 5Lm) was adopted as stationary 18 phase, the mobile phase consisted ofmethanol and water was used as gradient elute. Detector was DAD, the detection 1 wavelength was 293nm, flow ratewas 1. 0mL# m in . the column temperaturewas28e . R esults: The sample and acces- sory were separatedwell by HPLC. M etronidazole had a good linearity ( r = 0. 9995) in the range of 16.