HPLC法测定不同产地僵蚕中草酸铵含量.pdf

ModemBiomemcine2∞8v01．8No．5 ·915· 必生物医学进展w帆Lbiomed．net．阻P1．oFe8s量n ·药学· HPLC法测定不同产地僵蚕中草酸铵含量木 张琦严铸云△宋杰 陈新万德光 (成都中医药大学药学院中药材标准化实验室四川成都610075) 摘要目的：比较不同产地僵蚕和炒僵蚕中草酸铵的含量。方法：采用反相高效液相色谱法，色谱务件为色谱柱：Di锄。船il5斗C培 250x 4．6mmDikm；流动相：磷酸氢二铵水溶液(5g·L_1，pH (n=9)。结论：炮制僵蚕中草酸铵的含量普遍低于僵蚕生品；僵蚕生品中草酸铵的含量2．21％一5．82％，炮制品2．31％一5．78％。 关键词：僵蚕；草酸铵；高效液相色谱法 中图分类号：R931．6文献标识码：A文章编号：1673·6273(2008)05．0915-03 ofthe of DetemlinationContent0xalicAcidAmmonium仔omBomb、，)【 MoriLinnaeusininDif．ferentDistricts bVHPLC水 Jie，CHENXin。WAN Z琢蝌GQi。Ⅵ州动u—ytlll△，sONG De-guang． Chinese Medicimlstandardization Univefs毋oflhditio∞lMedicine，schoolofPhanmcy，ChineSe (Chengdu jal． Chen蓉du6l0075。Chim) ABSTRACT detenIlinetllec叩temofoxalicacid锄moni啪的m Li皿aeIlsinilldi仃ereIlt Objec6vc：To Bomby)【mori distric协by HPLCmethodwimacol咖ofDi锄onsil HPLC．Methods：A 4．6mmDikIm觚d solu- 5卜C塘250x ammoni啪m∞ohy赫c phosptlate tion(5 atO．5IIlL·m砒111e g‘L-1iIlpH5．30)髂memobilephasewereelnployed．ThenoW眦was∞t at214衄粕dc01urIlIl at30℃．kslll_b：Calibmti∞cllⅣew船liIleariIl of15．Ot0 wimnle temperatIlre me舢ge 150．0斗g·mL．1 re舯s- sioncoefficientof0．998．TIle contentofo】【alicacidammoniumin averagerecove巧w髂97．07％(RSD一2．67％，n=9)．Ccmchlsio璐：11le lowerman ones．TIlecontent is tlle o