一种在多糖分离纯化过程中新的脱蛋白方法_英文_王卫国.pdfVIP

hinese T raditional and Herbal Drugs34 10 2003 10 891 A novel approach to deproteinization in isolation and purification of lentinan from Lent inus ed od es 1 2 WANG Wei-guo , ZHAO Yong-liang ( 1. Department of Biology and hemical Engineering, Nanyang Institute of T echnology, Nanyang 473004, hina; 2. Key Biotechnology Laboratory Institute of Breeds and Resources, , hinese Agricultural Academy of Science and Technology, Beijing 100081, hina) : Abstract Object Looking for a suitable approach to deproteinization in isolation and purification of lentinan from L entin us edodes . MethodsT he orthogonal test was mainly used. ResultsA new approach was established for deproteinization in isolation and purification of a lentinan by orthogonal test. The re- sults indicated that the major factor affecting the deproteinization rate (DPR) was B ( 2-butanol) , the sub- major factor D (ethanol) ; while ( methanol) and E (Na l) had little influence. The influencing order for ( ) . 2- , DPR was B D A chloroform E DPR varied greatly when the amount of butanol varied going up slowly first, then going down rapidly. DPR decreased gradually as the concentration of ethanol in- creased. DPR went up first, and then went down, when the content of chloroform increased. And DPR al- tered little when the concentration of methanol increased. The optimal condition for deproteinization was 2 2 1 1 2 A B D E , the best composition for the deproteinizing agent was 40% of chloroform, 10% of 2-butanol, 2% of methanol, 40% of ethanol, 0.01% of Na l. Further proven experiments showed that DPR was up to 99.98% under the best condition. T he