反相高效液相色谱法测定豆制品中碱性橙.pdfVIP

2009 3 19 3 C inese Journal ofH ea lt Laboratory Tec nology, M ar 2009; Vol 19 No 3 545 = 反相高效液相色谱法测定豆制品中碱性橙 杨林飞 ( , 4 12000) [ ] : : + 011% ( 60: 40, V /V ), 454 nm : , A = 11774+ 219057( r = 01 9999), 01 02 ~ 45 L g/m ,l 01008 L g/m ;l RSD 1146% ~ 2128% ( n = 6) ; 9810% ~ 1001 8% : , , [ ] 碱性橙; 反相高效液相色谱法; 豆制品 + [ ] O65717 2 [] A [] 1004- 8685(2009) 03- 0545- 03 Determination of chrisodine in bean productsby reversed- phasehigh performance liquid chromatography YAN G L in-f e i ( Z uz ou Center for D isease Control and Prevention, Z uz ou 412000, C ina) [Abstract] Objective:A novel quantitativem et od for t e determ ination of c risodine in bean products on t e reversed- p ase ig perform ance liquid c rom atograp y as been developed1M ethods:M et anol- 011% form ic acid ( 60: 40, V /V) w as used as t e m ob ile p asew it K= 454 nm1Results:Under t e optmi al expermi ented conditions, t e regression equationw as A= 11 774+ 21 9057, t e linear range of c risod ine w as 01 02~ 45 Lg /m l w it r = 019999 and LOD = 01 008 Lg /m l1T e average recoveries w ere 9810% ~ 10018% w it R SD = 1146% ~ 21 28% (n = 6) 1Conclusion:T em et od is easy, fast, sens itive, reproducible and practical for t e analysis of c risod ine in bean products1 [ eywords] C risodine; Reversed- p ase ig perform ance liqu id c rom atograp ; Bean products , / 0, 1 [ 1] 111 仪器与试剂 ,