反相高效液相色谱法测定黄酒中的_苯乙醇.pdfVIP

※分析检测 食品科学 2009, Vol. 30, No. 14 175 反相高效液相色谱法测定黄酒中的β-苯乙醇 1 1 1 1 2 , 诸葛庆 ，李博斌 ，郑云峰 ，葛乐勇 ，刘兴泉 * ( 1. 国家黄酒产品质量监督检验中心，浙江绍兴 3 1207 1；2 .浙江林学院农业与食品科学学院，浙江 杭州 3 11300) 摘 要：建立一种采用反相高效液相色谱法测定黄酒中β- 苯乙醇的方法。采用反相C18 色谱柱Synergi Hrdro-RP C18 (4 .6m m ×2 50mm ，4 μm) ，以甲醇- 水为流动相(50 :50 ，V/ V) ，流速1ml/m in ，紫外检测器，检测波长2 10nm ， 对黄酒中的β- 苯乙醇进行检测。结果表明，在5.00～30.00mg/L 添加量范围内，回收率水平在99.5%～99.8% 之间， 相对标准偏差为0.6% (n = 6) ，方法的检出限为0.05mg/L ，线性范围为0.5～50mg/L(r = 0.9999) ，测定结果与标准 气相色谱方法基本相同。所建立的方法可以作为黄酒中β- 苯乙醇的检测方法。 关键词：反相高效液相色谱法；黄酒；β- 苯乙醇 HPLC Determination of Beta-Phenylethanol in Chinese Rice Wine 1 1 1 1 2, ZHUGE Qing ，LI Bo-bin ，ZHENG Yun-feng ，GE Le-yong ，LIU Xin g-quan * (1. National Center for Quality Supervision and Testing of Rice wine, Shaoxing 3 1207 1, China ； 2. School of Agriculture and Food Science, Zhej iang Forestry University, Hangzhou 3 11300, China) Abstract ：This study developed a reverse phase HPLC method for determing β-phenylethanol in Chinese rice wine. Beta- phenylethanol was separated on a Synergi Hrdro-RP C18 column (4.6 ×250 mm, 4 μm) using a mobile phase composed of CH3OH and H2O (50:50, V/ V) at a flow rate of 1 ml/min and detected using a UV detector set at 2 10 nm . At the spike levels of 5.00, 15.00 and 30.00 mg/L, the average recoveries for β-phenylethanol were between 99.5% and 99.8% with the RSDs of less than 0.6% (n = 3); the limit of detection was 1.0 ng, and the linear range was from 0.5 to 50 mg/L (r = 0.9999). The results of β- phenylethanol determination in four different rice wine samples by this method were almost the same a