复方牛膝口服液对卒中型自发性高血压大鼠的影响.docVIP

复方牛膝口服液对卒中型自发性高血压大鼠的影响 王丽君1,2 孙视越2 朱焰2 廖矛川1,2﹡ （1 中南民族大学，2 上海市计划生育科学研究所） [摘要] 目的：复方牛膝口服液对卒中型自发性高血压大鼠（SHRsp）的影响。方法：将SHRsp大鼠随机分为复方牛膝高、中、低剂量组，华佗再造丸组和病理组，每组10只，连续给药20天。观察复方牛膝口服液对SHRsp卒中后血压、神经状态变化、死亡率、脑系数及对海马区神经元的影响。结果：复方牛膝口服液可降低SHRsp大鼠血压，改善SHRsp脑卒中后的神经症状，延长卒中后存活时间，降低脑系数，降低脑卒中后大鼠死亡率，保护海马区神经元。结论：提示复方牛膝口服液对实验性SHRsp脑卒中有一定治疗作用。 关键词：复方牛膝口服液；卒中型自发性高血压大鼠（SHRsp）；脑卒中 Therapeutic Effects of Compound Achyranthes bidentata Oral Liquor(CAB) in SHRsp Wang lijun1,2 Sheng Zouyue2 Zhu Yan 2 Liao Maochuan＊1，2 (1.South-central University for Nationalities Wuhan 430074,China; 2.Shanghai Insitute of Planned Parenthood Research, Shanghai 200032,China ) [Abstract] Objective: To study the therapeutic effects of Compound Achyranthes bidentata Oral Liquor(CAB) in stroke-prone sponyaneously hypertension rats(SHRsp). Methods: Sixty SHRsp were randomly divided into five groups: CAB 1.0 g/kg 、 2.0 g/kg、4.0 g/kg ,Huatuo Zaizao Wan group(positive control group)2.5g/kg and pathologic group(n=10).CAB and Huatuo Zaizao Wan were used through filing stomach everyday for 20 days, pathologic group was given distilled water . The effects of CAB on blood pressure, changes of nerve state, death rate, brain index and the pathologic changes of hippocampal neuron of SHRsp were observed. Results: CAB could markedly decrease brain index, death rate of SHRsp after stroke. CAB could improve nerve state of SHRsp after stroke. Also CAB could prolong survival time and prevent pathologic change of hippocampal neuron of SHRsp after stroke. Conclusions: CAB is advantageous of therapying the stroke in SHRsp. . Keywords: Compound Achyranthes bidentata Oral Liquor; SHRsp; stroke. 1． 实验材料 1.1 动物 1.1.1 来源、种属、品纱：卒中型自发性高血压大鼠（SHRsp）大鼠，6周龄，雄性，由中国医学科学院阜外医院动物科提供，合格证号：医动字第01-1086号。 1.1..2 饲养条件：高血压大鼠专用饲料购自上海瑞金医院高血压研究所动物科，按纯系动物要求饲养，每笼5只，室温25±0.5℃，相对湿度50±10%，光照明暗各12小时。 1.2 受试物 复方牛膝口服液(以下简称：复方牛膝)：由上海市计划生育科研所植物化学室提供，批号 1.3 对照药物 华佗再造丸：广州奇星药业有限公司，批号：9911385 1.4 仪器 RBP-l型大鼠尾压心率测定仪，中日友好医院临床医学研究所 1.5 试剂 氯化钠：