大孔树脂纯化赤芍提取物的工艺考察.pdfVIP

第28 卷 第3 期 天津中医药大学学报 Vol．28 No．3 2009 年9 月 Journal of Tianjin University of Traditional Chinese Medicine Sep．2009 大孔树脂纯化赤芍提取物的工艺考察* 刘 虹，王 萌，郝 彧，朱晓月，拓小瑞，张德芹 摘要：[ 目的] 考察赤芍提取物的大孔树脂纯化工艺。 [方法] 以芍药苷为指标，进行了树脂型号的选择，考 察了上样量、洗脱溶剂的浓度及用量、树脂重复使用次数、再生后吸附力的恢复等。 [结果] 选择D101 大孔树 脂，上样量为树脂体积∶生药＝1∶1g(v/w)，洗脱溶剂4 倍量为70％ 乙醇。 [结论] 工艺经济简单实用，可以应用于纯 化赤芍提取物。 关键词：赤芍；大孔树脂；芍药苷 中图分类号： 文献标识码： 文章编号： （ ） R284.2 A 1673－9043 2009 03－0142-04 Study on the purification technology of radix paeoniae rubra extract by macroporous rresin LIU Hong, Wan Meng, HAO Yu, et al (Research institute of Tianjin University of TCM, Key Laboratory of Pharmacology of Traditional Chinese Medical Prescription, Ministry of Education, Tianjin 300193, China) Abstract: [Objective] To study the purification technology of Radix Paeoniae Rubra extract by macro- porous resin. [Methods] Taking peoniflorin as the index constituent, the type of resin was chosen. The sampling amount and elution solvent were decided and the times for repeated use and recovery capacity after regeneration were studied. [Results] D101 was chosen for purification, the suitable sampling ratio of resin volume to raw material was 1∶1. The elution solvent was 4-fold 70% ethanol. [Conclusion] The technology is economic, simple and practical and can be used to purify Radix Paeoniae Rubra extract. Key words: Radix Paeoniae Rubra; macroporous resin; peoniflorin 赤芍系毛茛科植物芍药（Paeonia lactiflora Pall.） 物制品检定所，批号110736-200525），赤芍药材（购 的干燥根。赤芍的药理作用广泛，可以抗凝、抗血 自亳州永刚饮片厂，经天津中医药大学中医药研究 栓、抗血小板聚集、对缺血性损伤有保护作用、抗动