小鼠骨髓和脾来源树突状细胞的分离与扩增培养.pdf

Journal of Henan Medical University　Mar.1999　Vol.34　No.1 ·19 · ＊ 1) 1) 1) 1) 2) 杨洪艳 　张义国 　董子明 　郑智敏 　吴　皓 1) 450052　2) 450052 　　　;;-;-4;;;; 　R392.12;Q28 　目的:(DC)。方法:BALB/C ;、CD4、CD8、B (McAb), ,T 、B , -DC ;-(GM-CSF)(IL-4) , DC DC (LC), 。结果:DC/LC , , ;, 1～4 , 95%。DC/LC (MLR)。结论:① DC/LC;②GM-CSF+IL-4 , DC/LC。 Isolation and urification of dendritic cells from mouse s leen and bone mar- row and their ex ansion culture induced by cytokines in vitro 1 ) 1) 1 ) 1 ) 2 ) Yang Hongyan , Zhang Yiguo ,Dong Ziming , Zheng Zhimin , Wu Hao 1 )Department of Pathophysiology ,Henan Medical University , Zhengzhou 4 00 2 　2 )Zhengzhou Railway Hospi- tal, Zhengzhou 4 00 2 　　Key words 　dendritic cells;cell culture;granulocyte-macro hage colony-stimulating factor (GM-CSF);interleukin-4;isolation; s leen;bone marrow;mouse Abstract 　Aims:To isolate and urify dendritic cells (DC)and their rogenitors from the BALB/C mouse s leen and bone mar- row in this ex eriment.Met hods:s leen or bone marrow cell sus ensions were re ared with ase tic technique, and then erythrocytes,T and B lym hocytes, granulocytes and monocyte-macro hages from them were removed se arately by treating successively with red cell lysing buffer, mouse anti-CD4 and anti-CD8 and anti-B220 monoclonal antibodies, and rabbit com lement buffer, and by semi-adhesion of DCs to culture lates.Existing DCs and their rogenitors were collected and induced in vitro culture by both granulocyte-macro hage colony-stimulating factor(GM-CSF)and interleukin-4 (IL-4).In mor hology DC were observed under inverted light microsco e and ex- amined by scanning electron microsco e.Results:In the course of the conditioned culture, the DC rogenitors differentiated and devel- o ed in