七氟醚预处理对大鼠海马脑片缺氧无糖损伤的保护作用线粒体KATP通道的作用.pdfVIP

生理学报 Acta Physiologica Sinica, June 25, 2006, 58 (3): 201-206 201 研究论文 七氟醚预处理对大鼠海马脑片缺氧无糖损伤的保护作用： 线粒体KATP 通道的作用 * 王志萍 ，张兆航，曾因明，江 山，汪曙蕖，王 胜 徐州医学院附属医院麻醉科，徐州医学院江苏省麻醉医学研究所，徐州221002 摘　要：利用离体海马脑片缺氧无糖(oxygen-glucose deprivation, OGD)损伤模型，探讨七氟醚预处理对神经细胞的保护作 用及该作用与线粒体内膜ATP 敏感钾通道(mitochondrial ATP-sensitive potassium channels, mitoKATP channels) 的关系，随机将 脑片用2% 、4% 、6% 七氟醚，以及6% 七氟醚复合mitoKATP 通道阻滞剂5- 羟基奎酸盐(5-hydroxydecanoic acid, 5-HD)预处 理30 min ，观察OGD 损伤14 min 复氧1 h 期间顺向群峰电位(orthodromic population spike, OPS)的变化，并应用透射电镜观 察细胞超微结构的改变。结果表明，与单纯OGD 组相比，七氟醚预处理可使海马脑片OPS 消失时间明显延长(P 0.01) ， 使OPS 明显恢复，其中4% 、6% 七氟醚组的恢复率均为71.4% (P0.05 vs OGD) ，相应恢复程度为(61.0 ± 42.3)% 和(78.7 ± 21.1)% (P0.01) ，而且6% 七氟醚的保护作用可被5-HD 取消。OGD 组的海马CA1 区锥体细胞明显水肿，核膜皱缩、破 裂，染色质聚集，线粒体肿胀畸形，嵴断裂或消失，而4% 和6% 七氟醚组仅见海马CA1 区锥体细胞轻度水肿，核膜皱 缩不明显，染色质均匀，线粒体轻度肿胀。结果提示，七氟醚预处理对大鼠海马脑片OGD 损伤有一定的保护作用，且 七氟醚对神经细胞的保护作用与激活mitoKATP 通道有关。 关键词：七氟醚；脑损伤；顺向群峰电位；线粒体内膜A T P 敏感钾通道 中图分类号：R6 14 .2 Protective effect of sevoflurane preconditioning on oxygen-glucose deprivation injury in rat hippocampal slices: the role of mitochondrial KATP channels * WANG Zhi-Ping , ZHANG Zhao-Hang, ZENG Yin-Ming, JIANG Shan, WANG Shu-Qu, WANG Sheng Department of Anesthesiology, Affiliated Hospital of Xuzhou Medical College, Jiangsu Province Institute of Anesthesiology, Xuzhou 221002, China Abstract: To investigate the neuroprotective effects of sevoflurane preconditioning on oxygen-glucose deprivation (OGD) injury and the role of mitochondrial KATP channels in rat, we established OGD injury model in rat hippocampal slices. The brain was rapidly removed, and the dissected hippocampus was sliced in cold artificial cerebrospinal fluid (ACSF) transversely to its longitudinal axis (400 µm thick) with a Rotorslicer DTY-7700. Slices were placed on a nylon mesh in a recording chamber at 34 °C and humidified gas mixture (95% O /5% CO ) was applied to the chamb