线粒体在铝致神经细胞凋亡中的调控作用_张勤丽.pdfVIP

　2007 33 2 Ind lth Occup Dis　2007, Vol. 33, No. 2 65 【】 线粒体在铝致神经细胞凋亡中的调控作用 张勤丽, 石樱桃, 王芳, 王林平, 张玲, 李秋营, 牛侨 (, 030001) 　　【】　。　0～3 d , , , 。　, 。, 、 , (P0.01)。Caspase , (P0.01, r 0.963, 0.859)。 C(CytC) , 3 CytC , (P0.05)。　 。 【】;;;; [] R 114　　　　 [] A　　　　[] 1000-7164(2007)02-006-5 06 Role of Mitochondria in A-l induced Neurocyte Apoptosis Z H A N G Qin-l i, S H I Ying-tao, WAN G F ang , WA N G L in-p ing , Z H A N G L ing , L I Qi u-y ing , N I U Qiao (School of Public ealth, Shanxi Medical University, Taiyuan Shanxi 030001 China) 　　【Abstract】Objective　To explore the role of mitochondria in A-l induced neurons apoptosis. Methods　 Neurons from 0 ～ 3 day newborn rats were cultured and treated with different concentration of aluminum chloride. Morphologic changes of neurons apoptosis and mitochondria were observed under light microscope and transmission electron microscope. R esults　Typical apoptosis morphologic characteristics of neurons were confirmed under light microscope and transmission electron microscope, and mitochondria were found swelling. The vitality of mitochondria enzyme in low dose group was found increased, but decreased in middle and high dose groups;the difference between high dose and control groups was significant. Active caspas-e 3 contents increased significantly in both cytoplasm and mitochondria as the aluminum concentration rose (P0.01, r=0.963, 0.859). CytC, which is localized only in mitochondria, was confirmed to be released into cytoplasm after aluminum treatment. The difference of CytC contents in 3 exposed groups was not significant. Conclusions 　Mitochondria struc