半矮生型桃生长跃变期差异表达基因的cDNA_AFLP初步分析.pdf

分子植物育种，2011 年，第9 卷，第1 期，第91-96 页 Molecular Plant Breeding, 2011, Vol.9, No.1, 91-96 研究报告 A letter 半矮生型桃生长跃变期差异表达基因的cDNA-AFLP 初步分析 1 1 1 2 1* 李钰婷 鲁振华 牛良 韩世明 王志强 1 中国农业科学院郑州果树研究所, 郑州, 450009; 2 江西省中国科学院庐山植物园, 庐山, 332900 * 通讯作者, wang6399@126.com 摘 要 半矮生型桃“SD9238”是在中国农业科学院郑州果树研究所育种圃实生苗后代中发现的突变体，其早 期生长缓慢，5 月中旬之后进入迅速生长期，与普通型截然不同。本研究以“SD9238”为研究试材，采用cDNA- AFLP 差显技术分析其新梢生长跃变期前后4 个生长阶段差异表达基因。研究中共采用256 对选择性引物组 合，扩增出9 021 条可分辨的条带，其中有差异的条带共有1 987 条。初步选取其中11 个差异条带，进行克隆 和序列测定，生物信息学分析表明，转录衍生片段(Transtript derived fragment, TDF) 的主要功能涉及木质素生 物合成；蛋白质水解；转录调控及生长调节等，还有一些是未知功能基因。研究所得结果为进一步揭示半矮生 桃生长调控的分子机理奠定了基础。 关键词 桃, 半矮生, cDNA-AFLP Preliminary Investigation of Differentially Expressed Genes Using cDNA-AFLP Approach During Growth Ramping of Semi-dwarf Peach Li Yuting 1 Lu Zhenhua 1 Niu Liang 1 Han Shiming 2 Wang Zhiqiang 1 * 1 Zhengzhou Fruit Research Institute, The Chinese Academy of Agricultural Sciences, Zhengzhou, 450009; 2 The Chinese Academy of Sciences, Lushan Botanical Garden, Lushan, Jiangxi Province, 332900 * Corresponding author, wang6399@126.com DOI: 10.3969/mpb.009.000091 Abstract The seedling mutant of “Semi-Dwarf 9238”was found in breeding pool of Zhengzhou Fruit Research Institute, the Chinese Academy of Agricultural Sciences, which grows slowly at the early stage, but grows fast after Mid-May. This trait is apparently different from that of standard type. We investigate the differentially expressed genes of SD9238 ’s new shoot at four stages using cDNA-AFLP approach. Two hundred and fifty-six primer com- binations were used for selective amplification. Nine thousand and twenty-one bands were generated, and one thousand nine hundred and eighty-seven TDFs were selected for their differencial expression. 11 TDFs were selected to be cloned and sequenced. The bioinformatic analysis indicate that the 11 TDFs putative present some significant similarities