基于 cox1基因全序列的癞蝗科部分类群系统发育关系分析.pdfVIP

Agricultural Science Technology, 2015, 16(2): 228-234 Copyright 訫 2015, lnformation lnstitute of HAAS. All rights reserved Molecular Biology and Tissue Culture Molecular Phylogeny of Pamphagidae (Acridoidea, Orthoptera) from China Based on Mitochondrial Cytochrome Oxidase l Sequences Weiwei SONG, Jinfeng HAO, Xiaoya ZHAO, Cuijiao WEN, Daochuan ZHANG * College of Life Sciences, Hebei University, Baoding 071002, China Abstract Phylogenetic relationships of Pamphagidae were examined using cy- 基 于 cox1 基因全序列的 癞 蝗 tochrome oxidase subunit l (cox1) gene sequences. Eleven species of Pamphagidae 科部分类群系统发育关系分析 from 7 genera were sequenced to obtain mtDNA data, along with 2 species from the GenBank nucleotide database. The results of sequence comparisons showed the 宋 卫 卫 ，郝 金 凤 ，赵 晓 雅 ，温 翠 娇 ，张 道 川 * cox1 gene is 1 534 bp in length, including 326 varible sites and 211 parsimonious information sites. The percentage of A +T is 67.1% in the nucleotide sequences, （河北大学生命科学学院 ，河北保定 071002） showing a strong AT bias. Genetic distances among subfamilies are 0.08. Using 摘 要 该研究测定了我国癞蝗科 7 属 11 种 Locusta migratoria as outgroup, the phylogenetic trees were reconstructed with NJ, 昆虫 细胞 色素 氧化 酶 I （cox 1）基因 全序 列 ，并 MP, ML and Bayesian inferences, and the results showed that the clustering results 联系从 GenBank 下载的贺兰短鼻蝗 、 红 缘疙 were approximately identical to that of classical morphological classification. 蝗的 cox 1 基因全序列进行序列分析 ， 结果表 Thrinchinae and Pamphaginae were a monophyletic group, respectively. Two species 明 ，除宽纹蠢蝗外 cox 1 基因全序列均为 1 534 of the genus Asiotmethis of Prionotropisinae did not get together with other species of Prionotropisinae, classification position of Asiotmethis should be further di