地衣芽孢杆菌16S-ITS标记及其特异性分析.pdf

ofAnhui 安徽农业科学，Journal 1，39(14)：8206—8207 责任编辑郑丹丹责任校对卢瑶 Agri．Sci．201 地衣芽孢杆菌16S-ITS标记及其特异性分析 陈冲1，佟建明2，张潞生3 农业大学农学与生物技术学院，北京100094) 以该区序列为靶序列设计地衣芽孢杆菌鸭旬1的特异性引物，并用该引物扩增全部受试茵种。[结果】从r偈和16SrDNA区间设计了地 衣芽孢杆菌种特异性引物，特异性引物PcR的最佳退火温度为67．2℃，循环数为24个；地衣芽孢杆菌聪-01可扩增出l条905bp的标 记片段。其他受试菌株均为阴性。从而证明试验得到了在种水平上对该菌种进行准确鉴定的特异16S—ITs标记。[结论]地衣芽孢杆茵 髑旬1分子鉴定方法的建立，为地衣芽孢杆菌的分子诊断奠定了基础。 关键词地衣芽孢杆菌；特异引物；】6S；I．TS 中图分类号S816．3文献标识码A 文章编号0517—6611(2011)14—08206—02 on16S-rrSMarkerfromBacillus andIts Analysis licheniformisSpecificity ClⅢN etal (InformationofShanxi 030801) Chong College A．gdculturalUniversity，Taigu，Shanxi Baz·；ffw AbstraetObiective】Theaimedtoestablishtheulolecularidentificationmethodsof the study licheniformis．[Method]皿lroughcloning testandthedifferenceon16SandIrIs of TS—01．the of TS-01was sequencing analysis sequencesB．Z／chen／form／s specificprimerB．Zichenlformis withthe oftheabove asthe andthewholetestedstrainswere this designed sequence regions targetsequence amplifiedbyusingspecificprimer。 Result1he were theDNAsectionof16SandfromthatofITSandthe annea— specificprimersof且Zicheniformisdesignedresp．from optimum forthe PCRwas67．2oCwiththe timesof TS-01could amarker of lingtemperaturespecificprimer cycle 24．B．1icheniformLsamplify fragment andothertestedstrainswereall thatthistestobtainedthe 16S—lTSmarkerthatcould this 905bp negative，thusproving specific accuratelyidentify strainatthelevelofthe