气相色谱-质谱联用法检测新生儿脐带血多环芳烃.pdfVIP

气相色谱．质谱联用法检测新生JLfl齐带血多环芳烃 郭勇勇¨，霍霞¨，吴库生”，陈爱明∞，彭琳I)，班皓¨，刘俊晓¨，徐锡金1) (1汕头大学医学院分析细胞学实验室，广东汕头515041；2美国辛辛那提大学医学院环境健康中心流行病学与生物统计学研究室，俄 亥俄州45267-0056。美国) 烷萃取；萃取的有机栩经SPE柱净化后，用气相色谱．电子电离源．质谱联用仪，选用离子检测法和外标法进行定性和定量。 儿脐带血中16种PAHs，灵敏度高、重现性好、回收率良好。 [关键词]多环芳烃；脐带血，新生儿；气相色谱．质谱；电子电离源 [中图分类号]R917 [文献标识码]A [文章编号]1007-4716(2010)02．0068．03 Cord Determinationof Aromatic inNewbornUmbilical Polycyclic HydrocarbOIlS BloodGas by Chromatography-ill_assSpectrometry GUO Xial，WU Ai—min2，PENGHnl，BANHa01，删Jun-xia01，xU Yong，yon91。HUOKu—shen91，CHEN Xi-jinl Division (1 CoZ拓ge，Shantol^‘515041C／dna，2 AM珈浏Cyt如SrLaboratory。S如ntou‰毋Medical 45267·0056，蚴) tics。DepartmentofEnvironmentalHealth，UniversiqofCindmuaiCoZtPgeofMedicine，ancimmti，OH methodfordeterminationof aromatic newborn a [Abstract]Objective：To polycyclic hy佻arbons(PAHs)in develop blood umbilicalcoldblood umbilicalcord bygaschromatography—tikesspectrometry(GC-MS)．Methods：Thesample withNa0Hin at60ccfor30 extractedwithn-hexaneinamber saponified EtOH．H20 minutes。was colored，capped comcal oftheextractWas SPE aheadofGC—MS withelectronicionization performed analysis flask．Cleanup by cartridges of additionWas73．0％-112．0％．Theof inselectiveion mode．Results：Thethestandard monitoring recovery recovery Was detectionlj觚tofthemethodWasinthe of0．03-4