水解大豆油催化复合酶制剂的研究.pdfVIP

ofAnhui 安徽农业科学，Journal Ash．Sci．2013，41(2)：819—820，852 责任编辑李菲菲责任校对李岩 水解大豆油催化复合酶制剂的研究 崔汝艳，王昌梅，张无敌+，陈玉保，尹芳，刘士清，柳静，赵兴玲(云南师范大学，云南昆明650092) 摘要[目的]得到能使大豆油彻底水解的复合脂肪酶配方。[方法]采用不同来源的脂肪酶对大豆油进行水解，以酸值的变化来衡量 水解程度，研究了单一脂肪酶和复合脂肪酶对大豆油的水解条件。[结果]单一脂肪酶水解时质量分数2％时水解最好，水解酸值达到 126 mgKOH／g油脂；使用复合脂肪酶时水解效率明显提高，达到150 mgKOH／g油脂左右。[结论]复合脂肪酶是一种既经济又很高效的 脂肪酶，它可使大豆油的水解率大大提高，并且克服了脂肪酶制备成本高、易失活、转化效率不太高等缺点，可以应用到工业上，降低成 本价，获得更大的利益。 关键词大豆油；复合脂肪酶；水解率 中图分类号S565．1文献标识码A 文章编号0517—6611(2013)02—00819—02 on of Oil Study Enzyme HydrolysisSoybeanCatalyzedbyComplex etal Normal CUIRu-yan(YunnanUniversity，Kunming，Yunnan650092) Abstract for of from sourceswas obtain different usedas to [Objective]Tocompoundlipasehydrolysissoybeanoil．[Method]Lipase catalyst ofacidvalueWasobservedfor the of the course．Theand degree experiment hydrolysissoybean．Thechange measuring hydrolysisduring single conditionsof were best conditionwas fractionof complexlipasehydrolysis lipase studied．[Result]Thehydrolysis themass 2％bysinglelipase． Theacidvaluecouldto126 to around150 compound up mgKOH／g．Usinglipasehydrolyze，theefficiencyobviouslyincreased，reaching an and couldbe in not can mgKOH／g．[Conclusion]Aseconomyhigh—efficiencylipase，thecompoundlipase appliedindustry，which only sig— oil alsocanovercome of costfor tolost nificantlyimprovesoybeanhydrolysisrate，but disadvantageshigh lipasepreparation，easy