中国大麦地方品种的遗传多样性及_淀粉酶活性的全基因组关联分析_姜晓东.pdfVIP

2013,46(4):668-677 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2013.04.002 α 1 2 100081 030801 ’ Analysis on the Genetic Diversity in Chinese Barley Landrace and Genomic Wide Association of α-amylase Activity Genes 2 1 1 JIANG Xiao-dong , GUO Gang-gang , ZHANG Jing 1 2 ( Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081; Agronomy College, Shanxi Agricultural University, Taigu 030801, Shanxi) Abstract: ObjectiveThe objective of this study is to understand genetic diversity in Chinese barley landraces and screen valuable molecular marker for α-amylase activity.Method A total 257 Chinese barley landraces were genotyped with 41 SSR markers distributing on seven chromosomes of barley. Nei’s genetic distance and neighbour-joining were adoped in cluster analysis. ResultA total of 709 alleles were identified at 41 SSR loci in 257 Chinese barley landraces. The number of alleles per locus ranged from 5 to 44, with an average of 17. The polymorphism information content value for SSRs ranged from 0.23 (Bmag 0385) to 0.94(Bmac0032) with a mean of 0.6385. Cluster analysis detected that nine clusters were identified based on Nei’s genetic distance in 257 accessions. Association analysis showed that five SSR markers significantly associated with α–amylase activity. Conclusion Chinese barley landraces have rich genetic diversity and could be clustered into nine different groups based on