河底沉积物培养耐酸产甲烷颗粒污泥的试验研究.pdfVIP

中国环境科学 2003,23(6)：587~591 China Environmental Science 河底沉积物培养耐酸产甲烷颗粒污泥的试验研究 * 凌雪峰,左剑恶,顾夏声 (清华大学环境科学与工程系厌氧生物实验室,北京 100084) 摘要：利用河底沉积物作为接种污泥,在一个3.1L 的EGSB 反应器中进行培养耐酸产甲烷颗粒污泥的试验研究.结果表明,EGSB 反应器在 3 pH6.0, 出水碱度低于400mg CaCO /L,容积负荷5.3kg COD/(m d)的条件下培养出具有良好沉降性能和产甲烷活性的耐酸颗粒污泥.形成耐 3 3 酸颗粒污泥后,EGSB 反应器在 pH5.8~6.0,进水 COD 3000mg/L,容积负荷 5.2kg COD/(m d)的条件下稳定运行 29d, COD 去除率平均为 89.2%,出水总碱度仅为264.4mg CaCO /L,沼气中甲烷的含量约为56.9%.扫描电镜观察发现颗粒污泥内部存在成簇生长的索氏甲烷丝菌. 3 关键词：颗粒污泥；酸性条件；河底沉积物；产甲烷 中图分类号：X522 文献标识码：A 文章编号：1000-6923(2003)06-0587-05 Studies on cultivation of acid-tolerant methanogenic granular sludge using river sediments. LING Xue-feng, ZUO Jian-e, GU Xia-sheng (Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China). China Environmental Science, 2003,23(6) ：587~591 Abstract：Studies on the cultivation of acid -tolerant methanogenic granular sludge in a 3.1L expanded granular sludge bed (EGSB) reactor using river sediments as seed sludge was conducted. In the reactor, acid-tolerant granular sludge with good settling ability and methanogenic activity was cultivated under the conditions of pH 6.0, effluent alkalinity lower 3 than 400mg CaCO /L, loading rate 5.3kg COD/(m d). Thereafter, under the condition of pH 5.8~6.0, influent COD 3 3 3000mg/L and loading rate 5.2kg COD/(m d), the reactor operated stably for 29 days with the average COD removal rate of 89.2%, effluent total alkalinity of 264.4mg CaCO /L and the methane content of about 56.9% in biogas . Investigated