季节变化对仓鼠褪黑素合成限速酶羟基吲哚-氧-甲基转移酶mRNA表达的影响.pdfVIP

季节变化对仓鼠褪黑素合成限速酶羟基吲哚-氧-甲基 转移酶mRNA表达的影响 徐颖 许筱颖郭霞珍 北京中医药大学，北京100029 摘要：目的：比较自然与人工模拟的四季对褪黑素合成限速酶羟基吲哚-氧-甲基转移酶(HIOMT)影响的 异同，为运用四时模拟系统模拟季节的可行性和可信性提供相关数据，进而为中医“天人相应”理论的研究提供新 的途径。方法：采用四季模拟系统人工模拟季节，测定模拟季节组和自然季节组仓鼠松果腺中褪黑素合成限速酶 HIOMTmRNA表达，并进行对比。结果：在自然条件下，仓鼠松果腺中HIOMTmRNA表达在冬季较高，冬季组和夏 季组相比有显著性差异(P＜0.05)，春季组和秋季组相比没有明显差异；在四季模拟系统条件下，模拟冬季组高 于模拟夏季组，且有显著性差异(P＜0.05)，春季组和秋季组相比没有明显差异，与自然条件下四季节律一致；模 拟四季组HIOMTmRNA表达与自然四季组同期相比均无显著性差异。结论：应用四季模拟系统模拟自然季节较为 成功，利用其模拟季节进行天人相应理论研究的思路具有可行性。 关键词：天人相应；四季模拟系统；羟基吲哚-氧-甲基转移酶mRNA;褪黑素 InfluencesofseasonalchangesonmRNAexpressionofrate-limitingenzymeHIOMTin thesynthesisofmelatonininhamsters XUYingXUXiao-yingGUOXia-zhen BeijingUniversityofChineseMedicine,Beijing100029,China Abstract:Objective:Weusedfourseasonsimulationsystemandartificiallysimulatednaturalseasonchangetocompare themRNAexpressionofrate-limitingenzymehydroxyindoleO-methyltransferase (HIOMT)innaturalseasonandsimulated naturalseason,analyzedthefeasibilityandcreditabilityofthefourseasonsimulationsystem.Itprovidedanewwaytoresearch correspondencebetweenmanandnaturetheory.Methods:Weusedfourseasonsimulationsystemandartificiallysimulatednatural seasonchangetoobservethemRNAexpressionofrate-limitingenzymeHIOMTinhamsterspinealglandinnaturalseason andsimulatednaturalseasonandcomparedtheresult.Results:Innaturalcondition,mRNAexpressionofpinealglandHIOMT presentedadispositiontendencythatitwashighinwinter.Therewassignificantdifferencebetweenthewinterandsummer groups(P＜0.05).Thedifferencebetweenspringandautumnwasnotsignificance.Infourseasonsimulationsystemcondition, mRNAexpressionofpinealglandHIOMTinthewintersimulationgroupwashigherthanthatinthesummersimulationgroup, withsignificantdifference(P＜0.05).Whilethedifferencebetweenspringandautumnwasnotsignificance,andwasconsistent withnaturalgroup.Therewereinsignificantdif