地沟油的钠离子检测法焰色反应.pdfVIP

※分析检测 食品科学 2011, Vol. 32, No. 12 213 1 , 2 1 1 1 1 , 2 魏益华 ，张金艳 ，戴廷灿 ，涂田华 ，罗林广 (1.江西省农业科学院农产品质量安全与标准研究所，江西 南昌 330200 ； 2.农业部肉及肉制品质量监督检验测试中心，江西 南昌 330200) 样品采用氧化钙为溶剂，在恒温马弗炉中 550 ℃加热4h ，冷却后用水超声浸提，过 On Guard Na Ⅱ SPE 柱除去处理液中金属离子，用离子色谱法测定其氯离子含量。结果表明：地沟油中氯离子含量远高于食 用油中氯离子含量。离子色谱条件：分析柱 IonPac AS19(4mm ×250mm) ，KOH 淋洗液 30.0mmol/L ，流速 1.0mL/min，进样体积500 μL ，外标峰面积定量。该方法对氯离子的检出限为0.004mg/L，在0.004～0.8mg/L 范围 内具有良好线性关系(r=0.9992) ，平均加标回收率为 88% ，相对标准偏差为(RSD)为 3.1% 。该法操作简便，快速 灵敏、准确。 离子色谱法；地沟油；食用油；氯离子 Determination of Chlorine in Hogwash Oil and Edible Oil by Ion Chromatography 1,2 1 1 1 1,2 WEI Yi-hua ，ZHANG Jin-yan ，DAI Ting-can ，TU Tian-hua ，LUO Lin-guang (1. Institute for Quality Safety and Standards of Agricultural Products, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China； 2. Supervision, Inspection and Testing Center for Quality of Meat-products, Ministry of Agriculture, Nanchang 330200, China) Abstract：An ion chromatography method was presented to determine chlorine in hogwash oil and edible oil. Oil samples were ashed in muffle furnace at 550 ℃for 4 h with calcium oxide, cooled down, extracted with water in an ultrasonic field, and purified on an On Guard Na Ⅱ SPE column for eliminating metal ions before ion chromatographic analysis. The chromatographic separation was carried out on an IonPac AS19 (4 mm × 250 mm) column using 30.0 mmol/L KOH as mobile phase at a flow rate of 1.0 mL /min. The injection volume was 500 μL. The external standard method was used to quantify chlorine. The detection limit of the method