胃癌患者外周血与胃癌组织中BRCA1基因甲基化的关系及其意义.pdfVIP

红 血与胃癌组织中BRCA1基因甲基化的关系区其意 胃癌患者外周血与胃癌组织中BRCA1基因甲基化的关系及其意义 徐红燕 ，王红兵 TherelationshipandsignificancebetweenmethylationofBRCA1geneinperipheralblood andingastriccancertissues Xu Hongyan ，WangHongbing XuzhouMedicalCollege，JiaugsuXuzhou221002，China；DepartmentofOncology，AffiliatedHospitalofXuzhouMedicalCollege，Jiang— SUXuzhou221002，China． 【Abstract】 0bjective：TodetectthepromotermethylationstatusofBRCA1geneingastriccancertissuesandcor— respondingperipheralblood，andtoexploretherelationshipandsignificancebetweenmethylationofBRCA1genepro— moterinperipheralbloodandingastriccancertissues．Methods：Methylation—specificPCR (MSP)wasperformed todetectthepromotermethylationstatusofBRCA1geneinthetumortissuesandthepairedperipheralbloodfrom37 gastriccancerpatients．Results：TherateofmethylationofBRCA1genepromoterwas40．5％ (15／37)inperipheral bloodsampleand48．6％(18／37)intissuesampleofgastriccancerpatients，andthecorrelationcoefficientwasr= 0．848(P=0．0004)．Conclusion：BRCAIgenepromotermethylationcanbedetectedinahighpercentageofthepe— ripheralbloodconsistingwiththatintumortissuesofgastriccancerpatients，andtherewasasignificantconcordance betweenperipheralbloodandtumortissuemethylationprofiles．ThedetectionofmethylationstatusofBRCA1gene promoterinperipheralbloodwassimple，fastandreliable，anditmaybeatargetforthetherapyofgastriccancer． K【eywords】gastriccancer；BRCA1；peripheralblood；methylation；methylationspecificPCR ModernOncology2015，23(02)：0244—0246 【摘要】 目的：检测胃癌患者外周血及胃癌组织中BRCA1基因启动子甲基化状态，并探讨两者的关系及意 义。方法：采用甲基化特异性PCR(methylation—specificpolymerasechainreaction，MSP)技术检测37例 胃癌患 者外周m及 胃癌组织中BRCA1基 启动子 甲基化状态。结果：胃癌患者外周血和 胃癌组织中BRCA1基因启 动子 甲基化率分别为40，5％(15／37)和 48．6％(18／37)，二者相关系数r=0．848(P=0．0004)。结论：胃癌患 者外周 与 胃癌组织中BRCA1基 启动子存在高比例的甲基化 ，而且两者 甲基化率有 良好的一致性。胃癌 患者外周血BRCA1基因启动子甲基化的检测具有