凡纳滨对虾血蓝蛋白抗肿瘤活性研究_陈传道.pdfVIP

2012 年中国水产学会学术年会论文摘要集 凡纳滨对虾血蓝蛋白抗肿瘤活性的研究 * 陈传道，黄润庆，路群山，章跃陵 （汕头大学理学院生物学系，广东省海洋生物技术重点实验室，广东 汕头 515063） 摘要：[目的] 探讨凡纳滨对虾血蓝蛋白的抗肿瘤活性及其可能的作用机制。[方法] 选用 SRB 和MTT 两种方法探索血蓝蛋白对 Hela 细胞的抗肿瘤活性；采用蛋白质组学、细胞生物学、分子生物学等的 技术分析血蓝蛋白对 Hela 细胞的细胞形态、细胞核以及蛋白表达的影响。[主要结果] 发现血蓝蛋 白处理 Hela 细胞 48h 后，对 Hela 细胞的抑制率为56.87-71.35%，其中有 11 个蛋白表达上调，6 个蛋白表达下调；血蓝蛋白能够使 Hela 细胞皱缩、变圆并产生类似阳性对照的凋亡小体；血蓝蛋 白还可以与 Hela 细胞细胞膜相互结合并能进入细胞内部，使细胞核基因组降解产生 100-300bp 左 右的梯状条带。[结论] 我们的研究首次发现血蓝蛋白具有抗肿瘤活性，其作用机制可能为：血蓝蛋 白以某种方式进入肿瘤细胞内部后，通过产生活性氧或醌类物质诱导肿瘤细胞以细胞程序性凋亡或 细胞自噬凋亡机制抑制肿瘤细胞生长。 关键词：对虾；血蓝蛋白；抗肿瘤活性 Investigations on the antitumor activity of hemocyanin from Litopenaeus vannamei Chuandao Chen; Runqing Huang; Qunshan Lu; Yueling Zhang * (Department of Biology and Guangdong Provincial Key laboratory of Marine Biotechnology, Shantou University, Shantou, Guangdong 515063) Abstract: [Objective] To explore the antitumor activity of hemocyanin from Litopenaeus vannamei and its molecular mechanism. [Methods] SRB and MTT assays were applied to explore the antitumor activity of hemocyanin on Hela cells. With the technology of proteomics, cell biology and molecular biology, the influence of hemocyanin on cellular morphology, nucleolus and protein expression level was analyzed. [Results] The growth of Hela cells treated with hemocyanin for 48h were strongly inhibited with the viability ranging from 56.87% to 71.35%. Compared with the contol group, there were11 up-regulated and 6 down-regulated protein spots. With the influence of hemocyanin, the Hela cells got shrinked and round with the appearance of apoptotic body-like vesicles. Furthermore, hemocyanin could bind to the cellular