半枝莲总黄酮脂质体的制备及包封率的测定.pdfVIP

北方药学 2015 年第 12 卷第 2 期 1 ·药品质量及检验· 半枝莲总黄酮脂质体的制备及包封率的测定 钱小娟 （江苏省泰州市人民医院南院病区药房 泰州 225300） 摘要： 目的：探讨半枝莲总黄酮脂质体的制备，建立半枝莲总黄酮含量测定方法，并测定其包封率。 方法：采用薄膜分散法来制备半 枝莲总黄酮脂质体，采用超速离心法分离脂质体及游离药物，采用紫外分光光度法（UV ）测定半枝莲总黄酮含量，并计算其包封 率。 结果：薄膜分散法制备的半枝莲总黄酮脂质体颜色为深褐色，呈圆形或类圆形，平均粒径为 0.54μm ，分布均匀，脂质体的平均 包封率为 94.38% ，符合 2010 版《中国药典》的要求。 结论：薄膜分散法可以简单快速地制备半枝莲总黄酮脂质体，操作流程掌握容 易，制备的脂质体形态较好、包封率较高；超速离心法与 UV 结合可以准确、可靠地测定半枝莲总黄酮脂质体的包封率。 关键词：半枝莲总黄酮 脂质体 制备方法 包封率 中图分类号：R286.0 文献标识码：A 文章编号：1672-8351（2015 ）02-0001-02 The preparation of Sculellaria barbata flavones Liposome and the determination of envelopment rate Abstract ：Objective ：TO discuss how to prepare the liposome of Sculellaria barbata flavones ，establish the method of total flavonoids determination ，and determate its encapsulation rate.Methods ：By using the film dispersion method to prepare the liposome of Sculellaria barbata flavones ，and by using the ultracentrifugation to liposome and free drugs ， and by using ultraviolet spectrophotometry （UV ）to determinate the total content of flavonoids ， and determate its encapsulation rate.Results ：The Liposome color is dark brown ，round or class round ，the average particle size was 0.54 microns ， uniform distribution ，and the average of liposome encapsulation rate is 94.38% ， it is in line with the requirements of “Chinese pharmacopoeia” 2010 version.Conclusion ：The film dispersion method is easy and simple to preparate the Sculellaria barbata flavones Liposome ，the operation is easy to grasp ，the morphology of liposomes prepared well and high encapsulation rate;The Ultracentrifugation method combined with UV can be accurate ， reliable to determinate of the encapsulation rate. Keywords ：Sculellaria barbata flavones Liposomes Preparation methods The encapsulation rate 半枝莲具有凉血解毒、散瘀活血、消肿