抗人卵巢癌单域噬菌体抗体地表达.pdfVIP

494 JOURNAL OF BEIJING MEDICAL UNIVER ITY Vol. 30 No. 6 Dec. 1998 抗人卵巢癌单域噬菌体抗体的表达* 周 萍 冯 捷 黄华梁 钱和年 蒋 欣 付天云 叶 雪 ( , 100034 ) / , 目的: COC183-B ( , V ) , 2 H 方法: V pFUW80 , H XL1-BLue, M 13 K07 , ELI A 结果: V H pFUW 80, , ELI A , 2. 5 结论: , R737. 31 EXPRESSION OF SINGLE DOMAIN PHAGE ANTIBODY OF ANTI-HUMAN OVARIAN CARCINOMA MONOCLONAL ANTIBODY # ZHOU Ping , FENG Jie, HUANG Hualiang, QIAN Henian, JIANG Xin, FU Tianyun, et al (# Gynecologic Oncology Center, People s Hospit al, Beijing Medical University, Beijing 100034) MeSH Ovarian neoplasms/ drug ther Bacteriophages Antibodies, monoclonal ABSTRACT Objective: To express the single domain ( heavy chain variable region gene, V ) antibody H of COC183-B ant-i human ovarian carcinoma monoclonal antibody for radioimmuno-imaging or target 2 therapy. Meth ds: After a secondary amplification of the V gene using a V -specific primer contain- H H ing X ho and Sp e sites, the products w ere digested w ith X ho and Sp e and then inserted into phagemid pFUW80 in the corresponding sites. The recombinants w ere then introduced into E . col i XL1-BLue cells. After phagemid rescue using M13KO7 as a helper phage, the single domain antibody w as expressed by phage-display. T he antigen binding activity of expressed product w as determined by ELI A. Results: The recombinants w ere digest