nbs-lrr全基因组分析.pdfVIP

Mol Gen Genomics (2004) 271: 402–415 DOI 10.1007/s00438-004-0990-z ORIGINAL PAPER T. Zhou Æ Y. Wang Æ J.-Q. Chen Æ H. Araki Æ Z. Jing K. Jiang Æ J. Shen Æ D. Tian Genome-wide identification of NBS genes in japonica rice reveals significant expansion of divergent non-TIR NBS-LRR genes Received: 28 September 2003 / Accepted: 10 February 2004 / Published online: 10 March 2004 Springer-Verlag 2004 Abstract A complete set of candidate disease resistance Keywords Rice Æ NBS-LRR genes Æ Molecular (R) genes encoding nucleotide-binding sites (NBSs) was diversity Æ Gene motif analysis Æ Disease resistance genes identified in the genome sequence of japonica rice (Oryza sativa L. var. Nipponbare). These putative R genes were characterized with respect to structural diversity, phy- Introduction logenetic relationships and chromosomal distribution, and compared with those in Arabidopsis thaliana. We Most plant disease resistance genes share similar se- found 535 NBS-coding sequences, including 480 non- quences and structural motifs (Kanazin et al. 1996). TIR (Toll/IL-1 receptor) NBS-LRR (Leucine Rich Re- These so-called R genes are thought to encode special- peat) genes. TIR NBS-LRR genes, which are common in ized receptors which recognize avirulence (Avr) gene- A. thaliana, have not been identified in the rice genome. dependent elicitors produced by pathogens (Keen 1990). The number of non-TIR NBS-LRR genes in rice is 8.7 When a plant receptor recognizes an elicitor, defense times higher than that in A. thaliana, and they account responses are activated that often lead to the death of for about 1% of all of predicted ORFs in the rice gen- the infected plant cell, thus preventing the pathogen ome. Some