不同碳源对金龟子绿僵菌IMI330189生长的影响.pdfVIP

30(1)58-64 中国生物防治学报 Chinese Journal of Biological Control 2014 年 2 月 不同碳源对金龟子绿僵菌IMI330189生长的影响 1,2 1,2 2 2* 王 磊 ，赵 霞 ，曹广春 ，张泽华 (1. 沈阳农业大学植物保护学院，沈阳 110161；2. 中国农业科学院植物保护研究所/农业部作物有害生物综合治理重点实验室, 北京 100081) 摘要：本文以单糖葡萄糖、果糖、蔗糖、葡萄糖＋果糖（1:1 ）、多糖可溶性淀粉以及可溶性淀粉经酶处理 后的水解产物为碳源，测定了发酵过程中糖消耗水平及绿僵菌生物量，分析了碳源对绿僵菌生长代谢的影 响。结果表明，供试碳源中，葡萄糖＋果糖的生物量最大（16.16 g/L ）、达到最大生物量时的增长速度也最 大（0.4238 g/L·h ），并且被绿僵菌利用最快（48 h ）；其它依次为蔗糖、葡萄糖、果糖、可溶性淀粉；相同 质量淀粉经外源α-淀粉酶水解12 h后作为碳源，与直接利用可溶性淀粉相比绿僵菌生物量增加14.3%左右。 由此可见多糖基质碳源不利于绿僵菌的吸收利用，若在多糖基质碳源中添加外源糖类水解酶提前将多糖降 解为单糖供绿僵菌生长，可提高绿僵菌对其利用率，进而提高发酵水平，降低发酵成本。 关 键 词：发酵；碳源；生物量；外源α−淀粉酶；成本 中图分类号：S476.12 文献标识码：A 文章编号：1005-9261 (2014)01-0058-07 Effect of Different Carbon Sources on the Growth of Metarhizium anisopliae IMI330189 1,2 1,2 2 2* WANG Lei , ZHAO Xia , CAO Guangchun , ZHANG Zehua (1. College of Plant Protection, Shenyang Agricultural University, Shenyang 110161; 2. Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China) Abstract: The effect of different carbon sources on the fermentation of Metarhizium anisopliae IMI330189 was determined in this paper. The effects of six carbon sources—glucose, sucrose, soluble starch, fructose, glucose＋ fluctose (1 :1) and the starch hydrolysate on the growth and the metabolism of M. anisopliae were discussed. The results showed that glucose ＋fluctose was the best carbon source among the six carbon sources, the biomass, growth rate and degradation time were 16.16 g/L, 0.4238 g/L·h, 48 h respectively, and the biomass were glucose ＋fluctose ＞sucrose＞glucose ＞fructose ＞soluble starch. The starch hydrolysate were f