白桦RAPD遗传连锁图谱的构建.pdf

HEREDITAS (Beijing) 2007 7 , 29(7): 867873 ISSN 0253-9772 DOI: 10.1360/yc-007-0867 RAPD , , , , , , , , , , 150040 : 80 (Betula pendula Roth)× (Betula platyphylla Suk) F1 2 10 F 1 1,200 10 bp , 208 RAPD , , 2 80 F1 , 364 χ2 307 1 1 , 26 3 1 , 31 145 , 162 2 , 145 14 (4 ), 6 6 , 37 , 955.6 cM (centimorgan), 14.9 cM 162 15 (4 ), 4 6 , 21 , 1,545.8 cM (centimorgan), 15.2 cM : ; ; DNA; ; ; Genetic linkage map of Betula pendula Roth and Betula platyphylla Suk based on random amplified polymorphisms DNA markers JIANG Ting-Bo, LI Shao-Chen, GAO Fu-Ling, DING Bao-Jian, QU Yue-Jun, TANG Xin-Hua, LIU Gui-Feng, JIANG Jing, YANG Chuan-Ping Key Laboratory of Forest Tree Improvement and Biotechnology of Ministry of Education, Northeast Forestry University, Harbin 150040, China Abstract: Based on the genetic inheritance and segregation of random amplified polymorphism DNA (RAPDs) markers, the first middensity linkage map for silver birch was constructed by using a pseudotestcross mapping strategy. A segregat- ing population including 80 progenies from the cross between Betula pendula Roth and B. platyphylla Suk was obtained. A set of 1,200 random oligonucleotide primers were screened, and 208 primers were selected to generate RAPD markers within a sample of 80 F1 progenies. A total of 364 segregating sites were identified. Among them, 307 belonged to 1 : 1 segregating site, and 36 belonged to 3 : 1 segregating site, others were found distorted from the nor