AbA对灰葡萄孢菌生长和繁殖的影响.doc

短梗霉素A对灰葡萄孢菌生长的影响( 赵红霞，苟萍*，刘小平，刘圣红，郭星军 (新疆大学生命科学与技术学院, 乌鲁木齐 830046) 摘要 通过考察短梗霉素A（AbA）对灰葡萄孢菌野生株BcAUR1及其AUR1基因内含子缺失突变株BcAUR1a生长的影响，明确AbA抑制真菌生长的机理。AbA敏感性试验表明，低浓度AbA（8 μg/mL）显著抑制野生株BcAUR1菌体的生长，高浓度AbA（50 μg/mL）存在下观察不到BcAUR1的生长迹象。突变株BcAUR1a则不受AbA的影响，在低浓度和高浓度AbA存在下均能正常生长。AbA抑制BcAUR1侵染柑橘果实，但BcAUR1a在高浓度AbA存在下也能够有效感染柑橘果实。这两个试验均证实了突变株BcAUR1a具有AbA抗性。电镜观察表明，AbA引起BcAUR1细胞质膜和内膜系统形态异常，质膜和液泡膜断裂，细胞内物质泄露。AbA抑制灰葡萄孢菌生长的机制是由于IPC合成酶受到抑制，导致鞘磷脂类物质合成不足，细胞膜结构破坏，胞内物质外漏。 关键词 灰葡萄孢菌；IPC合成酶；短梗霉素A；细胞形态 中图分类号：S188 文献标识码：A Effects of aureobasidin A on Botrytis cinerea’s growth Z Hongxia, Gou Ping*, LiuXiaoping, Liu Shenghong, Guo xingjun (College of Life Science and Technology, Xinjiang University, Urumqi 830046, China) Abstract In order to clarify the mechanism of Aureobasidin A（AbA）inhibiting the growth of fungal, the effects of AbA on growth of B. cinerea wild strain BcAUR1 and its intron of AUR1 gene deletion strain BcAUR1a were investigated. The experiments of AbA sensitivity showed that low concentration of AbA (8 μg/mL) significantly inhibited the growth of BcAUR1, and There had no sign of BcAUR1 growth under high concentrations of AbA (50 μg/mL). Howerer, AbA had no impact on BcAUR1a which can grow normally at low or high concentration of AbA. Additionally, AbA can inhibit infection of BcAUR1 to citrus fruit, but BcAUR1a can infect citrus fruits validly even at presence of high concentration of AbA. Both results confirmed that BcAUR1ahave a resistance to AbA. The observation of electron microscopy showed that AbA caused morphology abnormal of BcAUR1 cell membrane and inner membrane system, the fracture of plasmalemma and tonoplast, and the leakage of intracellular substances. The mechanisms that AbA inhibited the growth of B.cinerea is IPC synthetase was inhibited, which caused insufficient synthesis of sphingomyelin substances, damage of cells membrane structure and leakage of intracellular substances. Keywords Botrytis cinerea；inositol phosphatidyl ceramide (IPC) synthase；aureobasidin A