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Evaluation of the Performance and Intestinal Gut
©2007 Poultry Science Association, Inc.
Evaluation of the Performance and Intestinal Gut
Microflora of Broilers Fed on Corn-Soy Diets
Supplemented With Bacillus subtilis
PB6 (CloSTAT)1
A. Y. Teo and H.-M. Tan2
Research and Development Department, Kemin Industries (Asia) Pte Ltd.,
Singapore 758200, Singapore
Primary Audience: Veterinarians, Nutritionists, Researchers, Production Managers
SUMMARY
In this study, broilers provided feed containing 109 cfu/t Bacillus subtilis PB6 in the finisher
phase had a FCR similar to those on Zn bacitracin and significantly better than that of broilers
provided nonmedicated feed with no added B. subtilis PB6 (P 0.05). Over a 42-d period, broilers
provided feed with B. subtilis PB6 had comparable feed intake and FCR as the antibiotic control.
The counts of Lactobacillus species and Bifidobacterium species of broilers provided feed
supplemented with B. subtilis PB6 were not significantly different from the number of these bacteria
recovered from broilers provided feed supplemented with antibiotics. Numerically, up to 1- to 2-
log10 reduction in the number of Clostridium species recovered was observed in broilers provided
feed supplemented with B. subtilis PB6 when compared with both negative and antibiotic controls.
In terms of immunological response, birds provided feed supplemented with B. subtilis PB6 had
significantly heavier bursas, heterophils with higher in vitro phagoctyosis for Escherichia coli, and
lower ileal E. coli populations, indicating a potentiating role of B. subtilis PB6 as a probiotic on
the chicken innate immune system.
Key words: Bacillus subtilis, Lactobacillus species, Clostridium species, Escherichia coli, direct-fed
microbi
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