Fe对油脂积累的影响.pdfVIP

Chinese Journal of Oceanology and Limnology Vol. 32 No. 1, P. 47-53, 2014 /10.1007/s00343-014-3110-x Effect of Fe3+ on the growth and lipid content of Isochrysis galbana * 1 2, ** LIU Zhiyuan (刘志媛) , WANG Guangce (王广策) 1 College of Oceanology, Hainan University, Haikou 570228, China 2 Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China Received Mar. 20, 2013; accepted in principle May 29, 2013; accepted for publication Jun. 24, 2013 © Chinese Society for Oceanology and Limnology, Science Press, and Springer-Verlag Berlin Heidelberg 2014 Abstract Inducing lipid accumulation in microalgae cells without suppressing cell growth is vital to the economical production of biodiesel from microalgae. In two experiments, we demonstrate that the cell concentration and lipid content of marine microalgae Isochrysis galbana depend upon the iron concentration in the growth media. In Experiment I, adding chelated FeCl3 to the medium at the late exponential growth phase prolonged this phase and increased the lipid content in I . galbana cells. The fi nal cell density and lipid content of I . galbana supplemented with chelated FeCl3 was approximately 2 and 1.65 times higher than that of non-supplemented cultures, respectively. In Experiment II, I . galbana cells in the late exponential phase were collected and re-inoculated into new media containing Fe3+ at various concentrations. The fi nal cell concentration and lipid content were maximized at the highest iron concentration (38% biomass by dry weight at 1.2×10-5 mol/L Fe3+ ). In this study, intracellular neutral lipid storage was evaluated by fl uorescent spectrophotometry using fl uorochrome Nile red, and the measurement conditions were optimized. Keyword : biodiesel; cell density; iron; microalgae; Nile red 1 INTRODUCTION be effectively used