利用SSR分子标记分析茶树地方品种的遗传多样性.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2010, 36(12): 21912195 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2010.02191 利用SSR 分子标记分析茶树地方品种的遗传多样性 王丽鸳1 姜燕华1 段云裳2 成 浩1,* 周 健 1 曾建明1 韦 康 1 1 / , 310008; 2 , 210095 : 91 , SSR , SSR , , 15 , ; SSR , 90%SSR 5 , 24 90% : SSR ; ; ; Genetic Diversity of Tea Landraces Using SSR Markers 1 1 2 1,* 1 1 WANG Li-Yuan , JIANG Yan-Hua , DUAN Yun-Shang , CHENG Hao , ZHOU Jian , ZENG Jian-Ming , 1 and WEI Kang 1 Tea Research Institute, Chinese Academy of Agricultural Sciences / National Center for Tea Improvement, Hangzhou 310008, China; 2 Nanjing Agricultural University, Nanjing 210095, China Abstract: Appropriate assessment of genetic diversity in tea landraces is the prerequisite of conservation and utilization of these valuable genetic resources. Ninety-one individual tea plants, from Longjing tea landrace population, were used in this study. Com- puter simulation was utilized to study the changes of genetic diversity parameters influenced by the number of samples and alleles per SSR locus. The results showed that the sampling number differentially affected the genetic diversity parameters. Only when the sample number was higher than 15, the parameters tended to be stable. There were also large effects of the number of alleles per SSR locus on the genetic diversity, which was closely associated with the sample number. It was found when the number of alleles per SSR locus was five, at least 24 individual tea plants were needed for reaching to 90% of the to