发状念珠藻不同细胞破碎方法的研究.pdfVIP

现代食品科技 Modern Food Science and Technology 2009, Vol.25, No.11 发状念珠藻不同细胞破碎方法的研究 丁文杰，岳思君，郭伟，秦韶燕，贾士儒 (天津科技大学工业微生物教育部重点实验室, 天津 300457) 摘要：为了获得野生发状念珠藻和液态培养发状念珠藻细胞破碎和提取藻蓝蛋白的最佳方法，进行了发状念珠藻细胞破碎方法的 研究。采用液氮研磨、反复冻融，超声破碎，高压均质和玻璃珠破碎这几种方法对野生和液态培养发状念珠藻进行破碎研究。通过测 定破碎率、藻蓝蛋白提取得率和纯度，对几种细胞破碎效果和藻蓝蛋白提取方法进行比较。结果表明，采用高压均质方法能获得最佳 的破碎效果，当野生发状念珠藻和液态培养发状念珠藻细胞密度为 10 mg/mL 时，破碎率分别达到96.45 ％和97.06 ％。反复冻融法为 提取液态培养发状念珠藻藻蓝蛋白最理想的方法，其藻蓝蛋白的纯度和提取得率分别为 0.468 和 1.28 ％；高压均质为提取藻蓝蛋白的 最理想方法，其藻蓝蛋白纯度和提取得率分别为 0.153 和 1.43％。 关键词：藻蓝蛋白；细胞破碎；发状念珠藻 中图分类号：Q936；文献标识码：A ；文章篇号:1673-9078(2009)11-1243-04 Comparison of Different Cell Disruption Methods for Nostoc Flagelliforme DING Wen-Jie, YUE Si-jun, GUO Wei, QIN Shao-yan, JIA Shi-ru (Tianjin University of Science and Technology, Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin 300457, China) Abstract: In order to get the best method for extraction of C-phycocyanin from Nostoc flagelliforme, the cell disruption of N.flagelliforme were investigated using five methods, including liquid nitrogen grounding, repeated freezing and melting, ultrasonic disruption, high-pressure homogenization, and glass beads-breaking treatment. And the five methods were compared by determination the breakage rate, extraction rate and purity of C-phycocyanin. Results showed that high-pressure homogenization was the most efficient method for cell disruption, with which the breakage rates of wild and liquid-cultured Nostoc flagelliforme reached 96.45% and 97.06%, respectively, when their cell density was 10 mg/mL. And repeated freezing and melting method was the most suitable method for extraction of C-phycocyanin from liquid-cultured Nostoc flagelliforme , with which the extra