小麦与高冰草体细胞杂种后代的核基因组和叶绿体基因组的遗传特征.pdf

维普资讯 AetaBomn~aSinica 植 物 学 报 2(】()4，46(1)：110—115 http：Hwww．chinescplantscience．com HeredityofChloroplastandNuclearGenomesofAsymmetricSomatic HybridLinesBetweenW heatandCouchGrass CHENSui—Yun，LIU Shu—Wei，XUChun．Hui，CHENYong—Zhe，XIA Guang—Min (CollegeofLifeSciences，ShandongUniversity，Jinan250100，China) Abstract Chloroplastmicrosatelliteprimersofwheat(TriticumaestivurnL．)andrandomprimerswere employedtoidentifythechloroplastandnucleargenomesofthreeselfedlinesII-2，II—I一8(F2一F6)and8—1 (F3一F6)(segregatedfrom II—I一80fF2)，whichwerederivedfromthesameasymmetricsomatichybridclone betweenTriticumaestivumL．cv．Jinan177andAgropyronelongatum (Host)Nevski．Theresultsshowed thatthechloroplastgenomiccomponentsofthethreeIineswereconsistentanddominatedbythatof wheat．BandscharacteriStictobothparentswereonlydetectedinthesequenceoftheintergenicregion betweenrpll4andrpll6ofhtechloroplastgenome，suggestinghteexistenceofchloroplastDNA ofA． elongatum inthosehybrids．Furtherm0re．thisexogenousintegrationofchloroplastDNA waspassedtoF6 stably．RAPDanalysisshowedhtatthereweredi仟erentDNAfragmentsofA．elongatum jndifierentIines． However,thenucleargenomebasicallyremainedstableduringpassage． Keywords： somatichybridIinesbetweenTriticumaestivumandAgropyronelongatum：nucleargenome； chloroplastgenome；chloroplastmjcr0sateIIite：RAPD t Somatichybridizationisanewtechniqueforplantbreed— beenanalyzed，therebycpDNA patternsofmostsomatic ingduetoitspossibilitytocombinesexuallyincompatible hybridshasnotbeenacutallydefined．ThePCR—basedchlo— speciesnadbothhtenuclearna dcytoplasmicgenomesof roplastmi crosatellitemehtod