应用荧光原位杂交法检测乳腺癌石腊样本中.pdf

肿瘤防治研究 2007 年第 34 卷第 5 期 3·45 · 应用荧光原位杂交法检测乳腺癌石腊样本中 H ER - 2 基因的扩增 吕亚莉 , 钟 　梅 , 赵 　坡 Appl ication of Fluorescent in Situ Hybridization ( FISH) to Detect the Ampl if ication of HER2 Gene in Paraff in Sample of Breast Cancer L V Yali , ZHON G Mei , ZHAO Po D ep a rtment of P at hology , Chi nese PL A General H osp i t al , 2 8 F ux i ng R oa d , B eij i ng 100853 , Chi na Corresp on d i ng A ut hor : Z HA O Po , Em ai l :z haop o @30 1 hosp i t al. com. cn Abstract :Objective 　 To exp lore t he po ssibilit y of app lication wit h t he met ho d to det ect amplification of H ER2 gene by fluorescence in sit u hybridization ( F ISH) in clinical p at holo gy and molecular t ar geting t herap y in breast cancer , and investigat e t he relation ship between H ER2 gene amplification and clinico p at hological dat a . Methods 　Det ecting 50 cases of formalinfixed and p araffinembeded brea st duct al carci noma samples comp ared wit h result of immunohi stochemi st ry and t hen analyzed wit h clinicop at hological dat a . Results 　Sixt een of fift y (32 . 0 %) ca ses of breast carcinoma p resent ed po sitive for H ER2 p rot ein exp ression , including 5 st rong , 9 moderat e and 2 weak exp ression s , resp ectively . Eleven of fift y ( ) 22 . 0 % cases of brea st cancer showed po sitive for H ER2 gene amplification , in which 5/ 5 wa s t he ca ses showing st rongly po sitive and 6/ 9 p resenting mo derat ely po sitive exp ression for H ER2 p rot ein by immunohi stochemi st ry . And t he one wit h bot h amp lification of H ER2 gene and polysomy of chromo some 17 was al so moderat ely po sitive exp ression for t he p rot ein . Bot h gene amp lification and p rot ein ex p ression of H ER2 were corresponding to met ast a si s of lymp h nodes