无血清培养基与完全培养基体外诱导省略CIK细胞分泌细胞因子水平的比较蒋永新.pdfVIP

784 2006 33 11 CIK 蒋永新, 李高峰, 王熙才, 金从国, 李 佳, 陈晓群, 李 明, 伍治平 Comparison of Serum-Free Medium AIMV with Standard Serum-Containing Medium in Proliferation and Excretion Cytokine evel of CIK Cells in Vitro JIANG Yong-xin, LI Gao-f ng, WANG X-i cai, JIN Cong-guo, LI Jia, CHEN Xiao-qun, LI Ming, WU Zh-i ping Th ir d A f f iliated H osp ital of K unm ing M ed ical Colleg e ( Y unnan P r ov ince T umor H osp ital ) , K un- ming 650118, Ch ina Abstract:Objective T o compar s rum-fr m dium AIMV w ith standard s rum-containing m dium in prolif rat ion CIK c lls and x cr tion cytokin l v l in vit ro. Methods CIK c lls w r g n rat d by cultur CBMNCs in t h pr s nc of four cytokin s( IFN- , IL-2, IL- 1 and OKT 3) in s rum-fr m dium AIM V or in standard s rum-containing m dium s parat ly. Prolif ration, ph notyp s, x cr tion cytokin l v l w r compar d. Results Com par d w ith c lls cultur d in standard s rum-cont aining m dium, c ll cul- tur d in s rum-fr m dium AIMV th prolif ration p ak of CIK c lls w r lat r ( 14 ~ 17day) , but th prolif rat ion pow r w r high r ( 678. 7 tim ) . T h r w as no obvious diff r nc in prolif ration, ph no- t yp s, xcr t ion cytokin l v l of CIK c lls in t h tw o diff r nt m dia(P 0. 05) . Conclusion S rum-fr m dium AIMV may stand for standard s rum-containing m dium in prolif ration CIK c lls. Key words:S rum-fr m dium; Immunity ; C ll cultur ; Cytokin ; N oplasms : 比较无血清培养基( AIMV) 与完全培养基( CM ) 体外诱导扩增CIK 细胞及CIK 细胞分泌 细胞因子水平 分别用AIMV 及完全培养基加入4 种细胞因子( IFN-IL-2IL- 1 及OKT 3) 将 脐带血单 个核细胞( CBM NCs) 诱导成 CIK 细胞, 比较细胞的增殖能力细胞表型及分泌细胞因子水平 与完全培养基比较, 无血清培养基培养的CIK 细胞增殖高峰较晚( 14~ 17 天) , 但增殖倍数高, 在 细胞表型分泌细胞因子水平方面两种培养基培养的CIK 细胞均无明显的差别( P 0. 05) 无 血清培养基可代替完全培养基 : 无血清培养基; 免疫; 细胞培养; 细胞因子; 肿瘤 : R730. 45 : A : 1000- 8578( 2006) 11-0784-04 0 , CIK ( Cytokin -induc d kill r, CIK ) , , , , , ( AB , ,