《Effect of Semen vaccariae and Taraxacu mogono on Cell Adhesion of Bovine Mammary Epithelial Cells》.pdfVIP
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Availableonlineatwww.sciencedirect.com
■… ● ,J^ ■ ‘’r
匕trectOt5emenvaccanaeand,a尺jXaC£,noonCelIAdhesionofBovine
Cells
MammaryEpithelial
TONG andLI
Ye,LIU Xue-junQing-zhang
Jin-jin,LI Rong,GAO
150030,P.R.
ofLifeScience,NortheastAgriculturalUniversity,Harbin
KeyLaboratoryofDairyScience,MinistryofEducation/College
C^ina
andTaraxacuonthe
Theaimofthis istorevealthe mechanismoftheeffectofSemenvaccariae
study regulation mogono
cell.celladhersionmolecule.E.cadherinand onthe roleandsecretionfunctionofbovine
13-cateninproliferation mammary
cellsculturedin characterofbovine cellswasauthenticated
epithelial vitro.Firstly.theepithelial mammaryepithelial
immunofluorescence.thenthecell curvewasobservedand after&vaccariaeandZ
using grow investigated mogono
treatment.OntheeffectofSvaccariaeandr adhesionmolecules and 1
mogono,eell E—cadherin,B—cateninCycinD
mRNA weredetected andWestern resultsshowedthatthecellular
and byqRT-PCR blotting,respectively.The
protein
keratin18 and aftervaccariaeandZ mRNAand
expressedpositivelyprolifemedvigorously mogono仃eament.The
z
levelsofE—cadherinand 1 were 36haftervaecariaeand
protein CycinDremarkablyhigher(P.0.05)in mogono
treatment.Thecell at36hwasi
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