《Effect of serum on PEGylated quantum dots：Cellular uptake and intracellular distribution》.pdfVIP

Progress in Natural Science: Materials International 2013;23(6):566–572 Chinese Materials Research Society Progress in Natural Science: Materials International /locate/pnsmi ORIGINAL RESEARCH Effect of serum on PEGylated quantum dots: Cellular uptake and intracellular distribution Yeting Jian, Xianghui Xu, Yunkun Li, Zhongwei Gun National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Road, Chengdu 610064, China Received 26 September 2013; accepted 16 October 2013 Available online 5 December 2013 KEYWORDS Abstract Protein adsorption is closely related with the interactions between nanoparticles and physiological systems, and further influences the cellular uptake and distribution of nanoparticles in PEGylated QDs; Protein adsorption; cells. Although polyethylene glycol (PEG)ylation can largely reduce specific protein adsorption, some Cellular uptake; protein components in whole serum still interact with nanoparticles. In this work, PEGylated quantum Intracellular distribution dots (QDs) were used for investigating the quantitative and qualitative relationships of fetal bovine serum (FBS) and the cellular uptake/intracellular distribution in human hepatoma (HepG2) cell line. Nondenaturing polyacrylamide gel electrophoresis and two dimensional electrophoresis were used to analyze the adsorption of protein by PEGylated QDs. Quantitative cellular