《Effect of Sterilization Methods on Cell Binding Activity of Surfaceimmobilized Fibronectin》.pdfVIP

Biomed Environ Sci, 2014; 27(5): 391-395 391 Letter to the Editor Effect of Sterilization Methods on Cell Binding Activity of Surface-immobilized Fibronectin* 1 2 2 2 2,# XING Hai Bo , PAN Qin , WANG Kai Feng , LOU Fang , and LI Da The effect of sterilization methods on biological biomaterials[4]. Ultrafiltration is suitable for activity of fibronectin on the surface of biomaterials sterilizing biomolecules without radiating and was elaborated in the present study. Sterile deteriorating their molecular structure. However, it protein- modified biomaterials were fabricated by cannot be used for sterilizing the surface of microfilter filtration and UV irradiation, immobilized biomolecules because it is time respectively. UV irradiation altered the consuming and requires a sterile operation line. conformation of surface- adsorbed fibronectin and Generally, a specific sterilization method is selected further affected the attachment, morphology and based on economic considerations and implant biological function of endothelial cells. However, material properties. Most sterilization methods microfilter filtration did not to change the normal focus on their sterilization effect. However, their conformation of fibronectin, or the proliferation effect on the biological ac