《Effective Isolation of Retrotransposons and Repetitive DNA Families from the Wheat Genome》.pdfVIP
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Journal Plant 201
of
IntegrativeBiology0,52(7):679-691
Research
of
EffectiveIsolation and
RetrotranSpOSOnS
DNAFamiliesfrOmtheWheatGenome
MotonorlTomita’.MunenoriAsaoand Kuraki
Aya
MolecularGenetics of
Laboratory,FacultyAgriculture,TottoriUniversity,Tottori680·8553,Japan
author
‘Corresponding
85731
Tel(Fax):+815351:E·mail:tomita206@gmail.corn;tomita@muses.tottori·u.ac.JP
Availableonlineon25 2010at
May www.jipb.netand、^~vvv.interscience.wiley.com/journal/jipb
doi:10.11 1竹1744—7909.2010.00954.x
Abstract
Newclassesof DNAelementswere identified smalI ofthe
repetitive effectivelybyisolatingfragments
elementsfromthewheat wheatA wasconstructedfromTriticum
genome.AgenomeIibrary
withEc001
091.the sitesofwhichconsistedof
bydegeneratecleavage recognition
novel and derivedfromtheA
multi-sequences.Three
repetitivesequencespTm6.pTm69pTm58 genome
werescreenedandtestedfor numbera showed with
highcopy usingblottingapproach.pTm6identity
domainsofthe BA尺E-1and showed tothe
integrase barleyTyl-Copia-retrotransposOnpTm58 similarity
atandemwith
barleyTy3-gypsy-like arrayusefuI
didnotshare with
known elements.Thisalso
genomicspecificities,butanyidenti
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