《H2S Protecting against Lung Injury following Limb Ischemiareperfusion by Alleviating Inflammation and Water Transport Abnormality in Rats》.pdfVIP

410 Biomed Environ Sci, 2014; 27(6): 410-418 Original Article H S Protecting against Lung Injury following Limb 2 Ischemia-reperfusion by Alleviating Inflammation and Water Transport Abnormality in Rats* QI Ying Chun, CHEN Wen, LI Xiao Ling, WANG Yu Wei, and XIE Xiao Hua# Department of Comprehensive Surgery in South Building, Chinese People ’s Liberation Army General Hospital, Chinese People ’s Liberation Army Medical School, Beijing 100853, China Abstract Objective To investigate the effect of H S on lower limb ischemia-reperfusion (LIR) induced lung injury 2 and explore the underlying mechanism. Methods Wistar rats were randomly divided into control group, IR group, IR+ Sodium Hydrosulphide (NaHS) group and IR+ DL-propargylglycine (PPG) group. IR group as lung injury model induced by LIR were given 4 h reperfusion following 4 h ischemia of bilateral hindlimbs with rubber bands. NaHS (0.78 mg/kg) as exogenous H S donor and PPG (60 mg/kg) which can suppress endogenous H S production 2 2 were administrated before LIR, respectively. The lungs were removed for histologic analysis, the determination of wet-to-dry weight ratios and the measurement of mRNA and protein levels of aquaporin-1 (AQP ), aquaporin-5 (AQP ) as indexes of water transport abnormality, and mRNA and 1 5 protein levels of Toll-like receptor 4 (TLR ), myeloid differentiation primary-response gene 88 (MyD88) 4 a