《Histological and Physiological Investigation of Channelrhodopsin2 and Halorhodopsin in the Dorsal Cochlear Nucleus》.pdfVIP
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Journal 2011VoL6No.1
ofOtology
Article
Original
and of
HistologicalPhysiologicalInvestigation
and
Channelrhodopsin一2Halorhodopsin
inthe
DorsalCochlearNucleus
T.Shiman01‘27
Z.H.Panl.S.Molitor4.A.G.Holtl
J andCell State School
AnatomyBiologyDepartment,WayneUniversity
2 Medical
DepartmentofOtolaryngology,Kansai
3 Research School
KresgeHearing Institute,UniversityofMichiganofMedicine,Ann
4 Toledo,Toledo,OH,43606,USA
DepartmentofBioengineering,Universityof
AbstractWehavedeliveredviralvectors either fusedwith
containingChop2
or with form cationchannelsorchloride
Halorhodopsin(HaleR)fusedmCherry(tolightgated
intothedorsalcochlear to monthslaterweexaminedtheCNandinferior
nucleus(DCN).One
eighteen
(IC)forevidenceof transfeetedcellsand ofChR2andHaleRwasobserved
virally processes.Production
the localizationwithinneuronswas and cells
determined,with
DCN.Rhodopsin elongate,fusiformgiant
foundat the
basedon andlocationwithintheDCN.ProductionofChR2andHaloRwas both
morphology injection
siteaswellasin toandfromthe drivenneuronal intheDCNwas
regionsprojecting DCN.Light activity
the and o
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