乳酸菌抗热保护剂的优化组合.pdf

2010 7 CHINA CONDIMENT 35 1, 2 3* 程艳薇 , 谭书明 ( 1. ,550003; 2. , 550003;3. , 550003) :以干酪乳杆菌(L . casei) 鼠李乳杆菌和3 号菌为目标菌株, 对喷雾干燥乳酸菌粉抗热保护剂进行 4 了研究结 : 高温驯化后干酪乳杆菌鼠李乳杆菌和 3 号菌最高适应温度为50 , 通过L9 (3 )正交 试验,得出干酪乳杆菌高温驯化抗热保护剂最佳配比是在MRS 液体培养基中添加明胶1.5 g/ dL蔗糖 6 g/ dL甘油3 mL/ dL; 鼠李乳杆菌的最佳配比为: 明胶1 g/ dL蔗糖4 g/ dL甘油4 mL/ dL;3 号菌的 最佳配比为明胶 1.5 g/ dL蔗糖6 g/ dL甘油5 mL/ dL结论: 此条件下得到的复合抗热保护剂可以很 好的保护干酪乳杆菌鼠李乳杆菌和3 号菌的最佳存活率, 以满足将进一步进行的喷雾干燥法制备乳酸 菌粉发酵剂的需要, 所生产的乳酸菌粉的活性能够达到生产泡椒的要求, 进而缩短了泡椒发酵的时间, 保证了泡椒的良好风味 :干酪乳杆菌; 鼠李乳杆菌; 3 号菌; 抗热保护剂; 组合 :T S201. 57 :B : 1000- 9973(2010)07- 0055- 05 Optimization of lactic acid bacteria combination of heat- resistant protective agent 1, 2 3* CHEN Yan-wei ,TAN Shu-ming ( 1. uizhou University , uizhou Province Laboratory of Fermentation Engineering and Biological Pharmacy , uiyang 550003,China; 2. uizhou University, School of Chemistry and Chemical Engineering, uiyang 550003, China; 3. uizhou University, College of Life Science, uiyang 550003, China.) Abstract: L act obacil l us case i (L . casei) , rhamnose and 3 strains of Lactobacillus strains as the goal of spray-dried lactic acid bacteria powder heat-protective agent was studied. Results: After the high- temperature domestication , the highest temperature of Lactobacillus casei, Lactobacillus rhamnose 4