产木聚糖酶和纤维素酶真菌的酶学性质分析.pdf

食品研究与开发 2011 年9 月 生物工程 Food Research And Development 第32 卷第9 期 179 产木聚糖酶和纤维素酶真菌的酶学性质分析 麦国琴，许晓萍，余翠媚，张立国，王娟* （深圳大学生命科学学院，广东深圳 518060） 摘 要：红树林土壤微生物资源丰富，从深圳福田红树林土壤中分离了80株真菌，经过产酶发酵复筛，获得2株同时具 有较高木聚糖酶和纤维素酶活的真菌。通过菌株形态观察和分子鉴定，确定菌株1为草酸青霉（Penicillium oxalicum）， 菌株2为曲霉属的Aspergillus piperis 。菌株1的木聚糖酶和纤维素酶酶活分别为42.16 IU和3.05 IU，菌株2 的木聚糖酶和 纤维素酶酶活分别为39.01 IU和0.87 IU 。同时，对其进行酶学性质研究表明，2菌株木聚糖酶的最适温度均为50 ℃；菌 株1纤维素酶的最适温度是60 ℃，菌株2纤维素酶的最适温度是70 ℃，具有一定的耐热性。两菌株纤维素酶的最适pH 均为3.0；菌株1木聚糖酶的最适pH为5.0 ，菌株2木聚糖酶的最适pH为4.0 。 关键词：纤维素酶；木聚糖酶；土壤真菌；青霉；曲霉 Screening of Cellulase and Xylanase-pruducing Fungi from Mangrove Soil and Characterization of the Enzymatic Reactions MAI Guo-qin ，XU Xiao－ping ，YU Cui－mei，ZHANG Li－guo ，WANG Juan* （College of life sciences Shenzhen university ，Shenzhen 518060 ，Guangdong，China） Abstract: Mangrove ecological system has rich resources of microorganisms in the soil. Eighty strains of funfi were isolated from mangrove environment. Two strains of cellulase and xylanase-pruducing fungi were obtained after rescreening fermentation for enzyme production. The enzyme activities of cellulase and xylanase of strain No.1 is 42.16 IUand 3.05 IU respectively. The enzyme activities of cellulase and xylanase of strain No.2 is 39.01IU and 0.87 IU respectively. The optimum temperature of xylanase of two strains are all 50 ℃ . The optimum temperature of cellulase of strain No.1 and No.2 are 60 ℃ and 70 ℃ respectively. The optimum pH of cellulase of two strains are all pH3.0. The optimum pH of xylanase of strain No.1 and No.2 are pH5.0