利用RNA-Seq技术分析蒙古沙冬青低温胁迫表达谱及其AmNAC2基因的克隆.pdfVIP

Profile Cold under ExpressionAnalysis mongloicus ofAmmopiptanthus Stress andAmNAC2Gene byRNA-Seq Cloning Abstract Low canleadtoaseriesof inthe and temperature changes biochemical．and structurelevelsof whichthe in morphological plants，in changegene levelisan basisof andresistancetOlow expression importantgenetic plantresponse stress．Inthis of A temperature study，theexpressionprofile mmopipmnthus cold—resistant coldstresswas strong plant species，under analyzedbyRNA—Seqtechnology， andacold—induced factor namedAmNAC2Wascloned．The transcriptiongene mainresults areas follows， thelibrariesof0 hand24haftercold than8 (1)In h(contr01)，2h，8 stress，more millioncleanreadswere the each cleanreadsin were obtained,respectively．Afterlibrary totherefference of mapped unigenesequencesAmmopiptanthusmongloicustranscriptome assembledearlierin 61 wereidentified 568(71．5％)unigenes respectively． the levelsof inthecold—stressedlibraries (2)Throughcomparingexpressiongenes withthatincontrol 4413 7，and library,4038，391differentiallyexpr