Ca2+和茉莉酸对菠菜CBF表达的影响.pdf

中国震爹暹攉 2010，26(21)：59—62 Chinese ScienceBulletin Agricultural Ca“和茉莉酸对菠菜CBF表达的影响 于涌鲲1，赵福宽2，万善霞2，郝玉兰2，孙清鹏2 (1北京农学院组培试验中心，北京102206；2北京农学院生物技术系，北京102206) 摘要：为探究钙离子、茉莉酸(Jasmonic oleracea 达。推测低温可能首先导致细胞中JA含量升高，JA通过一定的机制诱导细胞质基质中游离钙离子浓 表达。 关键词：菠菜；Ca2+；茉莉酸；冷胁迫；CBF 中图分类号：Q942．6 文献标志码：A 论文编号：2010．1007 TheEffectofCalciumand Acidonthe ofCBFin Jasmonic ExpressionSpinach Yu Yongkunl，ZhaoFukuan2，WallShanxia2，HaoYulan2，Sun Qingpen92 102206； (1CenterofTissueCulture，Be{iingUniversityofAgriculture,Beijing 102206) DepartmentofBiotechnology,Be彬ngUniversityofAgriculture,Beijing ofthis istoevaluatetheeffectofcalciumand onthe ofCBF Abstract：，11le experiment JA expression objective in of weretreatedwithlow orA231 kindsof 87，one spinach．111eseedlingsspinach temperature(4℃)，JA calcium detectedthe ofCBFnorthern resultsshowed ionophorerespectively．thenexpressionby blotting．ne that CBF increasethe low andJA the may JA temperatureregulated expressionpositively．Lowtemperature contentofthecell inducedtheincreaseof calcium the JA JA firstly，then cytosolic inducedCa2+tOtransmitthelow CaMorCaMrelated the temperat