EST-SSR标记与茶树表型性状关联的初步分析.pdf

茶叶科学2010，30(1)：45-51 JournalTea oJ Science EST-SSR标记与茶树表型性状关联的初步分析 姚明哲，乔婷婷，马春雷，金基强，陈亮‘ (中国农业科学院茶叶研究所茶树资源与改良研究中心，国家茶树改良中心，浙江杭州310008) 摘要：关联分析是一种利用连锁不平衡(LD)检测自然群体中基因位点及其等位变异，并将等位基因变异与 因分型。在EST-SSR位点连锁不平衡和供试材料群体结构分析的基础上，通过关联分析共鉴定出5个与表型 CS338均与咖啡碱含量(CAF)显著关联，对表型变异的解释率分别为0．14和O．15。 关键词：茶树；EST-SSR；关联分析；连锁不平衡；群体结构 中图分类号：S571．1；Q348 文献标识码：A TheAssociationof Traitswith AnalysisPhenotypic E in ST-SSRMarkersTeaPlants YAO Chun—lei，JIN Ming．zhe，QIAOTing．ting，MA Ji．qiang，CHENLiang‘ CenterforTea (TeaResearchInstituteoftheChinese Sciences，NationalImprovement，Hangzhou310008，China) AcMemyofAgricultural molecularmarkers Abstract：Associationisan to the of orcandidate analysisapproachidentifyrelationship genes withtraitsina basedon this 55EST-SSRmarkers givenpopulationlinkagedisequilibrium(LD)．Instudy developed wereusedfor of1 12clonalteacultivars．LDof lociand structure independently genotyping pairwise population and7 traitswere were associationbetweenEST-SSRmarkers firstlyanalyzed，then analysis penotypic performed TASSELGLM．ThedifferentofLDweredetected55 EST-SSRloci．Geneticstructure using degrees amongpairwise showedthatthetotalof1 12teacultivarscanbeclassifiedintotwo EST-SSRmarkers analysis sub—populations．Five with traitswerescreenedoutatthe P0．01．ThreeMarkersCS298．CS3l7andC$84 associated level