不同来源丹参药材酚酸类成分的分析研究.pdfVIP

热带亚热带植物学报　2013 ，2 1(3) ：259~265 Journal of Tropical and Subtropical Botany 不同来源丹参药材酚酸类成分的分析研究 * 盛东峰, 李俐俐 (周口师范学院生命科学系， 河南 周口 466001) 摘要： 为 比较不 同来源的丹参(Salvia miltiorrhiza)药材的酚酸类成分 ，采用化学指纹图谱和定量分析的方法 ，对不 同来源丹参药 材 中的酚酸类成分进行 了系统分析。结果表 明：产地 、采收期 、病害、根色、根 的粗细以及药材部位等 因素尽管对丹参酚酸类成 分绝对含量 的影响比较大 ，但对各成分相对含量 的影响较小 ；不 同来源丹参药材酚酸类成分指纹 图谱相似性较高 ；8 月份采收 的药材 ，丹酚酸 B 含量较高 ；病害能够显著降低丹酚酸 B 的积累 ；与 白根和褐色根相 比，砖红色根 中的丹酚酸 B 含量较高 ；根越 粗 ，丹酚酸 B 含量也越高。这为丹参药材的品质评价和资源利用提供 了依据 。 关键词： 丹参 ；酚酸类成分 ；高效液相色谱 ；指纹图谱 ；质量评价 doi: 10.3969/j .issn.1005–3395.2013.03.012 Comprehensive Investigation of Phenolics in Salvia miltiorrhiza by HPLC Fingerprinting and Marker Compounds * SHENG Dong-feng , LI Li-li (Department of Life Science, Zhoukou Normal University, Zhoukou 466001, China) Abstract: In order to compare the phenolics in Salvia miltiorrhiza with different sources, the phenolics extracted from different sources were studied by chemical fingerprinting method, and salvianolic acid B as marker molecule was quantitatively analyzed. The results showed that the absolute amounts of phenolics in S. miltiorrhiza roots were seriously affected by different factors, such as origin, harvest time, root disease, root color, root diameter, and the part of plant. However, these factors had little influence on relative amounts of phenolics. The chemical fingerprinting of phenolics derived from different sources had high similarity. The content of salvianolic acid B harvested in August was the highest, and which was significantly reduced by diseases, such as root rot and nematode. Contents of phenolics in the brick red roots were the highest, compared to puce and white roots. The bigger the diameter, the higher contents of phenolics were. So, these could provide the basis for quality evaluation and resource utilization of S. miltiorrhiza. Key words: ; Phenolics; High performance liquid chromato