《Microbial Community Dynamics During Biogas Slurry and Cow Manure Compost》.pdfVIP

Journal of Integrative Agriculture 2013, 12(6): 1087-1097 June 2013 RESEARCH ARTICLE Microbial Community Dynamics During Biogas Slurry and Cow Manure Compost 1, 2* 1* 1 3 1 1 ZHAO Hong-yan , LI Jie , LIU Jing-jing , LÜ Yu-cai , WANG Xiao-fen and CUI Zong-jun 1 College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing 100193, P.R.China 2 Agronomy of Yanbian University, Yanji 133002, P.R.China 3 Alan G. MacDiarmid Research Institute of Renewable Energy, China Three Gorges University, Yichang 443002, P.R.China Abstract This study evaluated the microbial community dynamics and maturation time of two compost systems: biogas slurry compost and cow manure compost, with the aim of evaluating the potential utility of a biogas slurry compost system. Denaturing gradient gel electrophoresis (DGGE), gene clone library, temperature, C/N ratio, and the germination index were employed for the investigation, cow manure compost was used as the control. Results showed that the basic strip and dominant strips of the DGGE bands for biogas slurry compost were similar to those of cow manure compost, but the brightness of the respective strips for each system were different. Shannon-Weaver indices of the two compost systems differed, possessing only 22% similarity in the primary and maturity stages of the compost process. Using bacterial 16S rRNA gene clone library analysis, 88 bacterial clones were detected. Further, 18 and 13 operational taxonomic units (OTUs) were present in biogas slurry and cow manure compost, respectively. The 18 OTUs of the biogas slurry compost belonged to nine bacterial gene