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大孔吸附树脂纯化绵阳产绞股蓝总皂苷的研究
22 7 Vol. 22, No. 7
2010 7 Chem ical Research and Application Ju ly, 2010
: 100416 6( 2010) 0708840
*
罗娅君 , 李辉容, 帅小燕
(, 621000)
: ; ; ;
: O6 8 : A
Study on purification of gypenosides from Gynostemm a p en taphyllurn
( Thunb) M ak ino of m ianyang w ith m acroporou s absorp tive resin
*
LUO Yajun , L IHu irong, SHUA IX iaoyan
( Chem istry Chem ical Engineering college, M ianyang norm al University, M ianyang 621000, China)
A b stract: Using G insenoside Rb3 as a reference, the determ ination of Gypenosides from Gynostemm a pentaphyllurn ( Thunb)
M ak ino was perform edAccording to the elu tion rate and the purity of the products, the preparation perform ance of total sapon ins by
m acroporous resin was investigated, and its param eterswere optmi izedThe resu lts indicated that the Gypenosidesw as extractedw ith
60% ethanol by using ultrasound ( extracting rate w as 19 % , Gypenosides purity of Gypenosides w as 069 % ) The ratio of
1
diam eter to h ight ofm acroporous resin D101 was 18 The static absorption ration of macroporous resin D101 was 893mg g , the
1
disabsorption ration is 9383 % The dynam ic ration of m acroporous res in D 101 w as 322 m gg , the d isabsorption ration was
8679% The 80 % ethanol as eluen t, the con tent of Gypenos ides part eluted from D101 colum nw as 8961%
K ey w ords: Gynostemm a pentaphyllurn( Thunb)M ak ino; gypenos ides; m acroporous absorptive resin; absorption and pu rification
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