2-D Electrophoresis 二維電泳操作教學 - 銘傳大學.pptVIP

2-D Electrophoresis二維電泳操作教學 前言 第1章　二維電泳(2-D Electrophoresis)簡介 Core technique of proteomics Capable of simultaneously resolving thousands of proteins in one separation procedure Immobilized pH gradient gels: higher resolution improved interlaboratory reproducibility higher protein loading capacity extended basic pH limit Sorts proteins according to two independent properties in two discrete steps: the first-dimension step, isoelectric focusing(IEF), separates proteins according to their isoelectric pints(pI); the second-dimension step, SDS-polyacrylamide gel electrophoresis(SDS), sep